Human APO-SAA Active Protein – Cat# MBS691935

APO-SAA Human Active Protein

Name: APO-SAA Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS691935
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691935

Human Apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of Apo-SAA, normally 1-5 ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under… More details

$305 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Apo-SAA, Active Protein

Full Product Name

Human Apo-SAA

Product Gene Name

Apo-SAA active protein

Product Synonym Names

Recombinant Human Apo-SAA

Sequence

MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAA KRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG K DPNHFRPAG LPEKY

Host

E Coli

Species Reactivity

Human

Form/Format

Lyophilized (5mM Tris, pH7.8)

Purity/Purification

> 98% by SDS-PAGE and HPLC analyses

Preparation and Storage

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 6 months at -20°C to -80°C. Avoid repeated freeze and thaw cycles.

Molecular Weight

11.4 kDa

Chromosome Location

Chromosome: 11; NC_000011.10 (18266261..18269977). Location: 11p15.1

OMIM

104750

3D Structure

ModBase 3D Structure for P0DJI8

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin Level

< 0.1 ng /ug of protein (<1EU/ug).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containinga carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C

Length

104

Biological Activity

Tested by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 uM.

Product Categories/Family for Apo-SAA active protein

Cytokines & Growth Factors

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of Apo-SAA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Apo-SAA purchase

MBS691935 is an active protein targeting Apo-SAA. Also known as Human Apo-SAA. Reactive in Human. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Apo-SAA. It may not necessarily be applicable to this product.

NCBI GI #

40316912

NCBI GeneID

6288

NCBI Accession #

NP_000322.2

UniProt Primary Accession #

P0DJI8

UniProt Secondary Accession #

P02735; P02736; P02737; Q16730; Q16834; Q16835; Q16879; Q3KRB3; Q6FG67; Q96QN0; Q9UCK9

UniProt Related Accession #

P0DJI8

GenBank Nucleotide #

NM_000331.4

NCBI Official Full Name

serum amyloid A-1 protein preproprotein

NCBI Official Synonym Full Names

serum amyloid A1

NCBI Official Symbol

SAA1

NCBI Official Synonym Symbols

SAA; PIG4; SAA2; TP53I4

UniProt Protein Name

Serum amyloid A-1 protein

UniProt Synonym Protein Names

Amyloid fibril protein AA

UniProt Gene Name

SAA1

UniProt Synonym Gene Names

SAA

UniProt Entry Name

SAA1_HUMAN

NCBI Protein Information

serum amyloid A-1 protein; serum amyloid A protein; tumor protein p53 inducible protein 4

NCBI Summary for Apo-SAA

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

UniProt Comments for Apo-SAA

Function: Major acute phase reactant. Apolipoprotein of the HDL complex.

Subcellular location: Secreted.

Tissue specificity: Expressed by the liver; secreted in plasma. Ref.18

Induction: Upon cytokine stimulation.

Post-translational modification: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Polymorphism: At least 5 different SAA1 alleles have been described: SAA1.1 (SAA1alpha), SAA1.2 (SAA1beta), SAA1.3 (SAA1gamma), SAA1.4 (SAA1delta), SAA1.5 (also named SAA1beta but which differs from SAA1.2). We use here the revised nomenclature described in Ref.21. The sequence shown is that of SAA1.1.

Involvement in disease: Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Ref.14Elevated serum SAA1 protein levels may be associated with lung cancer. Ref.14

Sequence similarities: Belongs to the SAA family.

Mass spectrometry: Molecular mass is 11702±14 Da from positions 19 - 122. Determined by MALDI. Ref.18Molecular mass is 11682.7 Da from positions 19 - 122. Determined by MALDI. Ref.22Molecular mass is 11526.5 Da from positions 20 - 122. Determined by MALDI. Ref.22Molecular mass is 11439.6 Da from positions 21 - 122. Determined by MALDI. Ref.22Molecular mass is 11363.6 Da from positions 20 - 121. Determined by MALDI. Ref.22Molecular mass is 11235.6 Da from positions 20 - 120. Determined by MALDI. Ref.22Molecular mass is 10872.6 Da from positions 22 - 119. Determined by MALDI. Ref.22Molecular mass is 8337.5±0.8 Da from positions 19 - 94. Determined by ESI. With variants Ala-70, Val-75, Asn-78 and 86-Leu-Thr-87. Ref.14Molecular mass is 8390.9±0.2 Da from positions 19 - 94. Determined by ESI. With variant Ala-70. Ref.14

Research Articles on Apo-SAA

1. All amino acid residues in the SAA sequence, essential for the formation of the protein complex with cystatin C, are basic ones.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Apo-SAA active protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies	Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
Amyloids Pathway antibodies	Amyloids Pathway Diagram
Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies	Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies	Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies	DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Folate Metabolism Pathway antibodies	Folate Metabolism Pathway Diagram
Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies	Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram

Diseases associated with Apo-SAA active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>101 publications with Apo-SAA and Inflammation
Necrosis Antibodies	>31 publications with Apo-SAA and Necrosis
Neoplasms Antibodies	>31 publications with Apo-SAA and Neoplasms
Kidney Diseases Antibodies	>20 publications with Apo-SAA and Kidney Diseases
Liver Diseases Antibodies	>19 publications with Apo-SAA and Liver Diseases
Cardiovascular Diseases Antibodies	>14 publications with Apo-SAA and Cardiovascular Diseases
Disease Models, Animal Antibodies	>13 publications with Apo-SAA and Disease Models, Animal
Liver Neoplasms Antibodies	>12 publications with Apo-SAA and Liver Neoplasms
Atherosclerosis Antibodies	>12 publications with Apo-SAA and Atherosclerosis
Carcinoma, Hepatocellular Antibodies	>11 publications with Apo-SAA and Carcinoma, Hepatocellular

Organs/Tissues associated with Apo-SAA active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>80 publications with Apo-SAA and Liver
Lung Antibodies	>14 publications with Apo-SAA and Lung
Skin Antibodies	>3 publications with Apo-SAA and Skin