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Lefty-1 (LEFTY1) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS692431

Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and… More details

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Product Overview

Product Name
Lefty-1 (Lefty1), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse Lefty-1
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2
Clone Number
12A13
Host
Rat
Species Reactivity
Mouse
Form/Format
Lyophilized
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C.
Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Molecular Weight
41,498 Da
3D Structure
ModBase 3D Structure for Q64280
Reconstitution
Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml.
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Lefty-1. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Lefty1 antibody
Western Blot (WB)

Other Notes

Small volumes of anti-Lefty1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Lefty1 purchase

MBS692431 is an antibody targeting Lefty1. Also known as Rat Anti-Mouse Lefty-1. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Lefty1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Left-right determination factor 1
NCBI Official Synonym Full Names
left right determination factor 1
NCBI Official Symbol
Lefty1
NCBI Official Synonym Symbols
Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052
UniProt Protein Name
Left-right determination factor 1
UniProt Synonym Protein Names
Protein lefty-1; Lefty protein; Stimulated by retinoic acid gene 3 protein; Transforming growth factor beta-4; TGF-beta-4
UniProt Gene Name
Lefty1
UniProt Synonym Gene Names
Ebaf; Lefty; Stra3; Tgfb4; Lefty protein; TGF-beta-4
NCBI Protein Information
left-right determination factor 1

NCBI Summary for Lefty1

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning. [provided by RefSeq, Aug 2016]
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UniProt Comments for Lefty1

LEFTY1: Required for left-right axis determination as a regulator of LEFTY2 and NODAL. Belongs to the TGF-beta family.

Protein type: Cytokine; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1|1 H4

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; transforming growth factor beta receptor binding

Biological Process: anterior/posterior axis specification; cell development; cell migration involved in gastrulation; determination of left/right symmetry; heart morphogenesis; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of MAPKKK cascade; response to retinoic acid
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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