• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

APRIL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695417

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL. Anti-Human APRIL specific antibody was purified by affinity chromatography employing immobilized hAPRIL matrix. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
APRIL, Polyclonal Antibody
Full Product Name
Anti-human APRIL
Product Gene Name
anti-APRIL antibody
Product Synonym Names
Anti-Human APRIL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified
Preparation and Storage
At -20 degree C
Molecular Weight
36,589 Da
OMIM
604472
3D Structure
ModBase 3D Structure for O75888
Immunogen
Recombinant Human APRIL
Applications Tested/Suitable for anti-APRIL antibody
Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-APRIL antibody
Immunohistochemistery: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.

Sandwich ELISA: To detect hAPRIL by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human APRIL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAPRIL.

Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-APRIL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APRIL purchase

MBS695417 is an antibody targeting APRIL. Also known as Anti-human APRIL. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APRIL. It may not necessarily be applicable to this product.

NCBI GI #
13124605
NCBI GeneID
8741
NCBI Accession #
O75888.1
UniProt Primary Accession #
O75888
UniProt Secondary Accession #
Q541E1; Q5U0G8; Q96HV6; Q9P1M8; Q9P1M9; A8MYD5; B4DVT2
UniProt Related Accession #
O75888
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 13
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13
NCBI Official Symbol
TNFSF13
NCBI Official Synonym Symbols
APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13
UniProt Synonym Protein Names
A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD_antigen: CD256
Protein Family
Tumor necrosis factor ligand superfamily
UniProt Gene Name
TNFSF13
UniProt Synonym Gene Names
APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1
UniProt Entry Name
TNF13_HUMAN
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2

NCBI Summary for APRIL

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
Show more

UniProt Comments for APRIL

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol; external side of plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; gene expression; signal transduction; positive regulation of isotype switching to IgA isotypes
Show more

Research Articles on APRIL

1. Our results suggest that an elevated level of BAFF/APRIL drives the maturation of B cells, subsequently leading to IgA2 class switching, and possibly to the development of interstitial lung disease in patients with mixed connective tissue disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APRIL antibody

 Reference Product  PubMed Publications
 TNFSF13B antibody  >95 publications with APRIL and TNFSF13B
 TNFRSF17 antibody  >60 publications with APRIL and TNFRSF17
 TNFRSF13B antibody  >45 publications with APRIL and TNFRSF13B
 SET antibody  >8 publications with APRIL and SET
 FURIN antibody  >5 publications with APRIL and FURIN

Pathways associated with anti-APRIL antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HuR Stabilizes MRNA Pathway antibodies  HuR Stabilizes MRNA Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway antibodies  Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-APRIL antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >216 publications with APRIL and Necrosis
 Inflammation Antibodies  >20 publications with APRIL and Inflammation
 Disease Models, Animal Antibodies  >13 publications with APRIL and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >11 publications with APRIL and Cardiovascular Diseases
 Colorectal Neoplasms Antibodies  >11 publications with APRIL and Colorectal Neoplasms
 Nervous System Diseases Antibodies  >10 publications with APRIL and Nervous System Diseases
 Kidney Diseases Antibodies  >6 publications with APRIL and Kidney Diseases
 Liver Neoplasms Antibodies  >5 publications with APRIL and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >5 publications with APRIL and Neoplasms, Experimental
 Liver Diseases Antibodies  >4 publications with APRIL and Liver Diseases

Organs/Tissues associated with anti-APRIL antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >163 publications with APRIL and Blood
 Bone Antibodies  >21 publications with APRIL and Bone
 Spleen Antibodies  >16 publications with APRIL and Spleen
 Lung Antibodies  >11 publications with APRIL and Lung
 Kidney Antibodies  >10 publications with APRIL and Kidney
 Lymph Node Antibodies  >9 publications with APRIL and Lymph Node
 Skin Antibodies  >7 publications with APRIL and Skin
 Liver Antibodies  >6 publications with APRIL and Liver
 Vascular Antibodies  >5 publications with APRIL and Vascular
 Mouth Antibodies  >3 publications with APRIL and Mouth
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000