Chicken IFN-alpha ELISA Kit – Cat# MBS027651

Interferon Alpha (IFN-alpha) ELISA Kit for Chicken

Name: Chicken IFN-alpha ELISA Kit; Interferon Alpha; MyBioSource
Brand: MyBioSource
SKU: MBS027651
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS027651

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IFN-alpha concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken IFN-alpha antibody to coat Microelisa… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interferon alpha (IFN-alpha), ELISA Kit

Full Product Name

Chicken Interferon alpha ELISA Kit

Product Gene Name

IFN-alpha elisa kit

Species Reactivity

Chicken

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

3.12 pg/ml - 100 pg/ml.

Sensitivity

1.0 pg/ml.

Specificity

No significant cross-reactivity or interference between Chicken IFN-alpha and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IFN-alpha concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken IFN-alpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IFN-alpha and IFN-alpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of IFN-alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFN-alpha purchase

MBS027651 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon alpha (IFN-alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFN-alpha. The ELISA analytical biochemical technique of the MBS027651 kit is based on IFN-alpha antibody-IFN-alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IFN-alpha. It may not necessarily be applicable to this product.

Protein Family

Interferon alpha/beta receptor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.