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Fibroblast Growth Factor 23 (FGF23) Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2097280
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MBS2097280 is an active protein targeting FGF23. Also known as Active Fibroblast Growth Factor 23 (FGF23). Reactive in Mouse. Host: E Coli. More details

$215 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor 23 (FGF23), Active Protein
Full Product Name
Active Fibroblast Growth Factor 23 (FGF23)
Product Gene Name
FGF23 active protein
Product Synonym Names
ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor
Host
E Coli
Species Reactivity
Mouse
Form/Format
Freeze-dried powder
Purity/Purification
> 90%
Molecular Weight
Predicted Molecular Weight: 28.7kDa
Accurate Molecular Weight: 29kDa
3D Structure
ModBase 3D Structure for Q9EPC2
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Tyr25~Val251
Isoelectric Point
9.8

Product Categories/Family for FGF23 active protein

Cytokine; Tumor immunity; Infection immunity; Genetic science
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for FGF23 active protein
Cell culture, Activity Assays

Activity Data of FGF23 active protein

FGF23 (Fibroblast growth factor 23) is a member of the fibroblast growth factor family, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF23 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF23. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF23 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF23 for 48h. The result was shown in Figure 2. It was obvious that FGF23 significantly increased cell viability of 3T3 cells.
Mouse FGF23 Active Protein – Cat# MBS2097280

SDS-Page of FGF23 active protein

Mouse FGF23 Active Protein, SDS-Page – Cat# MBS2097280

Testing Data of FGF23 active protein

Mouse FGF23 Active Protein, Testing Data – Cat# MBS2097280

Testing Data of FGF23 active protein

Mouse FGF23 Active Protein, Testing Data – Cat# MBS2097280

Other Notes

Small volumes of FGF23 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FGF23. It may not necessarily be applicable to this product.

NCBI GI #
12083589
NCBI GeneID
64654
NCBI Accession #
NP_073148.1
UniProt Primary Accession #
Q9EPC2
UniProt Related Accession #
Q9EPC2
GenBank Nucleotide #
NM_022657.4
NCBI Official Full Name
fibroblast growth factor 23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
Fgf23
UniProt Protein Name
Fibroblast growth factor 23
Protein Family
Fibroblast growth factor
UniProt Gene Name
Fgf23
UniProt Synonym Gene Names
FGF-23
NCBI Protein Information
fibroblast growth factor 23

NCBI Summary for FGF23

This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function. [provided by RefSeq, Mar 2013]
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UniProt Comments for FGF23

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6|6 F3

Cellular Component: extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding; type 1 fibroblast growth factor receptor binding

Biological Process: cell differentiation; cellular phosphate ion homeostasis; fibroblast growth factor receptor signaling pathway; MAPK cascade; negative regulation of bone mineralization; negative regulation of hormone secretion; negative regulation of osteoblast differentiation; phosphate ion homeostasis; positive regulation of transcription, DNA-templated; regulation of bone mineralization; vitamin D catabolic process
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Research Articles on FGF23

1. deleting FGF23 within early osteoblasts and osteocytes demonstrated that both cell types contribute to baseline circulating FGF23 concentrations, and that targeting osteoblasts/osteocytes for FGF23 production can modify systemic responses to changes in serum phosphate concentrations and rescue the Hyp genetic syndrome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FGF23 active protein

 Reference Product  PubMed Publications
 Fgfr1 active protein  >58 publications with FGF23 and Fgfr1
 Fgfr4 active protein  >21 publications with FGF23 and Fgfr4
 Fgfr3 active protein  >17 publications with FGF23 and Fgfr3
 Fgfr2 active protein  >12 publications with FGF23 and Fgfr2
 Fgf8 active protein  >7 publications with FGF23 and Fgf8
 Fgf9 active protein  >7 publications with FGF23 and Fgf9
 Fgf18 active protein  >5 publications with FGF23 and Fgf18
 Fgf20 active protein  >4 publications with FGF23 and Fgf20
 Fgf17 active protein  >2 publications with FGF23 and Fgf17

Pathways associated with FGF23 active protein

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR1 Pathway antibodies  Downstream Signaling Of Activated FGFR1 Pathway Diagram

Diseases associated with FGF23 active protein

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >576 publications with FGF23 and Kidney Diseases
 Phosphorus Metabolism Disorders Antibodies  >318 publications with FGF23 and Phosphorus Metabolism Disorders
 Rickets Antibodies  >312 publications with FGF23 and Rickets
 Renal Insufficiency Antibodies  >273 publications with FGF23 and Renal Insufficiency
 Hyperphosphatemia Antibodies  >184 publications with FGF23 and Hyperphosphatemia
 Calcinosis Antibodies  >136 publications with FGF23 and Calcinosis
 Hypertension Antibodies  >108 publications with FGF23 and Hypertension
 Hypertrophy Antibodies  >61 publications with FGF23 and Hypertrophy
 Death Antibodies  >58 publications with FGF23 and Death
 Inflammation Antibodies  >45 publications with FGF23 and Inflammation

Organs/Tissues associated with FGF23 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >608 publications with FGF23 and Blood
 Skin Antibodies  >35 publications with FGF23 and Skin
 Spleen Antibodies  >6 publications with FGF23 and Spleen
 Pancreas Antibodies  >5 publications with FGF23 and Pancreas
 Inner Ear Antibodies  >1 publications with FGF23 and Inner Ear
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