DDAH1 Recombinant Protein – Cat# MBS2009339

Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) Recombinant Protein

Name: DDAH1 Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS2009339
Price: 200 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2009339

MBS2009339 is a recombinant protein targeting DDAH1. Also known as Recombinant Dimethylarginine Dimethylaminohydrolase 1 (DDAH1). Host: E Coli. More details

$200 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Dimethylarginine Dimethylaminohydrolase 1 (DDAH1), Recombinant Protein

Full Product Name

Recombinant Dimethylarginine Dimethylaminohydrolase 1 (DDAH1)

Product Gene Name

DDAH1 recombinant protein

Sequence

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A LPESLGQHAL RSAKGEEVDV ARAERQHQLY VGVLGSKLGL QVVELPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG AEILADTFKD YAVSTVPVAD GLHLKSFCSM AGPNLIAIGS SESAQKALKI MQQMSDHRYD KLTVP

Host

E Coli

Form/Format

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Purity/Purification

> 95%

Preparation and Storage

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Matching Pairs

Unconjugated Antibody: DDAH1 (MBS2002194)
Immunogen: DDAH1 (MBS2009339)

Matching Pairs

APC-CY7 Conjugated Antibody: DDAH1 (MBS2073766)
Immunogen: DDAH1 (MBS2009339)

Molecular Weight

25.2kDa

Chromosome Location

Chromosome: 1; NC_000001.10 (85784168..86044046, complement). Location: 1p22

OMIM

604743

3D Structure

ModBase 3D Structure for O94760

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Organism

Homo sapiens (Human)

Expression System

Prokaryotic expression

Residues

Ala20~Pro215 (Accession # O94760) with two N-terminal Tags, His-tag and T7-tag

Endotoxin Level

<1.0EU per 1ug (determined by the LAL method)

Predicted Isoelectric Point

5.9

Reconstitution

Reconstitute in sterile PBS, pH7.2-pH7.4.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for DDAH1 recombinant protein

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Other Notes

Small volumes of DDAH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DDAH1. It may not necessarily be applicable to this product.

NCBI GI #

197313763

NCBI GeneID

23576

NCBI Accession #

NP_001127917.1

UniProt Primary Accession #

O94760

UniProt Secondary Accession #

Q5HYC8; Q86XK5

UniProt Related Accession #

O94760

GenBank Nucleotide #

NM_001134445.1

NCBI Official Full Name

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 2

NCBI Official Synonym Full Names

dimethylarginine dimethylaminohydrolase 1

NCBI Official Symbol

DDAH1

NCBI Official Synonym Symbols

DDAH; HEL-S-16

UniProt Protein Name

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

UniProt Synonym Protein Names

DDAHI; Dimethylargininase-1

Protein Family

N(G),N(G)-dimethylarginine dimethylaminohydrolase

UniProt Gene Name

DDAH1

UniProt Synonym Gene Names

DDAH; DDAH-1

UniProt Entry Name

DDAH1_HUMAN

NCBI Protein Information

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAHI; DDAH-1; dimethylargininase-1; epididymis secretory protein Li 16; NG, NG-dimethylarginine dimethylaminohydrolase

NCBI Summary for DDAH1

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]

UniProt Comments for DDAH1

DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.3.18

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: mitochondrion

Molecular Function: amino acid binding; dimethylargininase activity; metal ion binding; catalytic activity

Biological Process: positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; regulation of systemic arterial blood pressure; arginine catabolic process; nitric oxide mediated signal transduction

Research Articles on DDAH1

1. Elevated asymmetric dimethylaginine is not a part of the proatherogenic risk profile in the young adult offspring of patients with premature Coronary artery disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DDAH1 recombinant protein

Reference Product	PubMed Publications
NOS3 recombinant protein	>6 publications with DDAH1 and NOS3
PRMT1 recombinant protein	>2 publications with DDAH1 and PRMT1

Diseases associated with DDAH1 recombinant protein

Disease Name	Pubmed Publications
Hypertension Antibodies	>12 publications with DDAH1 and Hypertension
Heart Diseases Antibodies	>10 publications with DDAH1 and Heart Diseases
Disease Models, Animal Antibodies	>8 publications with DDAH1 and Disease Models, Animal
Nervous System Diseases Antibodies	>8 publications with DDAH1 and Nervous System Diseases
Brain Diseases Antibodies	>7 publications with DDAH1 and Brain Diseases
Fibrosis Antibodies	>5 publications with DDAH1 and Fibrosis
Atherosclerosis Antibodies	>4 publications with DDAH1 and Atherosclerosis
Kidney Diseases Antibodies	>3 publications with DDAH1 and Kidney Diseases
Hypertrophy Antibodies	>3 publications with DDAH1 and Hypertrophy
Immune System Diseases Antibodies	>2 publications with DDAH1 and Immune System Diseases

Organs/Tissues associated with DDAH1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>28 publications with DDAH1 and Vascular
Heart Antibodies	>24 publications with DDAH1 and Heart
Lung Antibodies	>12 publications with DDAH1 and Lung
Brain Antibodies	>12 publications with DDAH1 and Brain
Kidney Antibodies	>10 publications with DDAH1 and Kidney
Muscle Antibodies	>9 publications with DDAH1 and Muscle
Liver Antibodies	>7 publications with DDAH1 and Liver
Embryonic Tissue Antibodies	>4 publications with DDAH1 and Embryonic Tissue
Nerve Antibodies	>3 publications with DDAH1 and Nerve
Uncharacterized Tissue Antibodies	>2 publications with DDAH1 and Uncharacterized Tissue