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CGB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9214030

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological… More details

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Product Overview

Online Datasheet in PDF
Product Name
CGB/HCG-Beta, Polyclonal Antibody
Full Product Name
CGB/HCG-Beta Antibody (C-term)
Product Gene Name
Product Synonym Names
Choriogonadotropin subunit beta; CG-beta; Chorionic gonadotrophin chain beta; CGB; CGB3
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This CGB/HCG-Beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human CGB/HCG-Beta.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
Molecular Weight
17739
Immunogen Sequence Positions
137-165
OMIM
118860
3D Structure
ModBase 3D Structure for P01233
Function
Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Cellular Location
Secreted.
Tissue Location
High expression in the placenta throughout pregnancy.
Applications Tested/Suitable for anti-CGB antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-CGB antibody
WB~~1:1000
IHC-P~~1:10-50
E~~Use at an assay dependent concentration

Western Blot (WB) of anti-CGB antibody

Anti-CGB/ HCG-Beta Antibody (C-term)at 1:2000 dilution + human placenta lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CGB Polyclonal Antibody – Cat# MBS9214030

Western Blot (WB) of anti-CGB antibody

CGB/HCG-Beta Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CGB/HCG-Beta antibody detected the CGB/HCG-Beta protein (arrow).
Rabbit Anti-CGB Polyclonal Antibody, Western Blot (WB) – Cat# MBS9214030

Immunohistochemistry (IHC) of anti-CGB antibody

CGB/HCG-Beta Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CGB/HCG-Beta Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CGB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9214030

Western Blot (WB) of anti-CGB antibody

All lanes : Anti-CGB/HCG-Beta Antibody (C-term)at 1:500 dilution
Lane 1:Ramos cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L),
Peroxidase conjugated at 1/15000 dilution.
Observed band size :34kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CGB Polyclonal Antibody, Western Blot (WB) – Cat# MBS9214030

Western Blot (WB) of anti-CGB antibody

All lanes : Anti-CGB/HCG-Beta Antibody (C-term)at 1:1000 dilution
Lane 1:Human placenta lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.
Observed band size :34kDa
Blocking/Dilution buffer: 5% NFDM/TBST
Rabbit Anti-CGB Polyclonal Antibody, Western Blot (WB) – Cat# MBS9214030

Product References and Citations for anti-CGB antibody

Cole, L.A. Placenta 31(8):653-664(2010) Ra, Y.J., et al. Interact Cardiovasc Thorac Surg 11(1):114-116(2010) Verma, B., et al. J. Immunol. 184(4):2156-2165(2010) Handschuh, K., et al. Placenta 30(12):1016-1022(2009) Reisenbichler, E.S., et al. Breast J 15(5):527-530(2009)

Other Notes

Small volumes of anti-CGB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CGB purchase

MBS9214030 is an antibody targeting CGB. Also known as CGB/HCG-Beta Antibody (C-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CGB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
choriogonadotropin subunit beta
NCBI Official Synonym Full Names
chorionic gonadotropin, beta polypeptide
NCBI Official Symbol
CGB
NCBI Official Synonym Symbols
CGB3; CGB5; CGB7; CGB8; hCGB
UniProt Protein Name
Choriogonadotropin subunit beta
UniProt Synonym Protein Names
Chorionic gonadotrophin chain beta
Protein Family
UniProt Gene Name
CGB
UniProt Synonym Gene Names
CGB3; CG-beta
UniProt Entry Name
CGHB_HUMAN
NCBI Protein Information
choriogonadotropin subunit beta

UniProt Comments for CGB

CGB: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Belongs to the glycoprotein hormones subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; apoptosis; peptide hormone processing; female gamete generation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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