Nicotinamide Phosphoribosyltransferase (NAMPT) ELISA Kit for Rat

Introduction: Visfatin, an adipokine that is highly enriched in the visceral fat, has increased expression level in plasma during the development of obesity, implying its important role in obesity. Visfatin (also known as PBEF and Nampt), a 52 kDa cytoplasmic protein composed of 473-amino acid, expresses primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue. In addition to its role in obesity, visfatin also has other biological functions. Visfatin is a nicotinamide phosphoribosyltransferase (Nampt) enzyme that catalyzes first step in the biosynthesis of NAD from nicotinamide. Visfatin has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for visfatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain visfatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of visfatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of NAMPT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700290 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAMPT. The ELISA analytical biochemical technique of the MBS700290 kit is based on NAMPT antibody-NAMPT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAMPT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAMPT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).