Rudimental Bovine Serum Albumin Check-Up ELISA Kit for Rat

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BSA. Standards and samples are pipetted into the wells with biotin-conjugated antibody preparation specific for BSA. A competitive inhibition reaction is launched between BSA (Standards or samples) and pre-coated BSA with the biotin-conjugated BSA antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of BSA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of rudimental bovine serum albumin check-up elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700338 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the rudimental bovine serum albumin check-up, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing rudimental bovine serum albumin check-up. The ELISA analytical biochemical technique of the MBS700338 kit is based on rudimental bovine serum albumin check-up antibody-rudimental bovine serum albumin check-up antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect rudimental bovine serum albumin check-up antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, rudimental bovine serum albumin check-up. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).