Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase) (MMP2) ELISA Kit for Mouse

Introduction: 72 kDa type IV collagenase also known as matrixmetalloproteinase-2 (MMP-2) and gelatinase A is an enzyme thatin humans is encoded by the MMP2 gene.Proteins of the matrix metalloproteinase (MMP) family are involvedin the breakdown of extracellular matrix in normal physiologicalprocesses, such as embryonic development, reproduction, andtissue remodeling, as well as in disease processes, such asarthritis and metastasis. Most MMP's are secreted as inactiveproproteins which are activated when cleaved by extracellularproteinases. This gene encodes an enzyme which degrades typeIV collagen, the major structural component of basementmembranes. The enzyme plays a role in endometrial menstrualbreakdown, regulation of vascularization and the inflammatoryresponse

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then addedto the appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for MMP-2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate welland incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine)substrate solution is added to each well. Only those wells thatcontain MMP-2, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of MMP-2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

Small volumes of MMP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700408 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) (MMP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP2. The ELISA analytical biochemical technique of the MBS700408 kit is based on MMP2 antibody-MMP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).