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Geminin (GMNN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2401732

Geminin is a protein that plays a critical role in cell cycleregulation. the encoded protein inhibits DNA replication by binding toDNA replication factor Cdt1, preventing the incorporation ofminichromosome maintenance proteins into the pre-replication… More details

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Product Overview

Product Name
Geminin (GMNN), Polyclonal Antibody
Full Product Name
Anti-Geminin Antibody (aa50-100)
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 2H7] (IgG1) to Human Geminin; GMNN; Geminin
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Specificity
Endogenous levels of human geminin protein.
Preparation and Storage
Store lyophilized at -20 degree C.
The reconstituted product can be stored for short term at 4 degree C or long term at -20 degree C or below.
Avoid freeze/thaw cycles.
Molecular Weight
23,565 Da
OMIM
602842
3D Structure
ModBase 3D Structure for O75496
Immunogen
KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
Conjugation
Unconjugated
Epitope
aa50-100
Resuspension Liquid
Distilled water
Applications Tested/Suitable for anti-GMNN antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-GMNN antibody
ELISA: 0.05-0.2 ug/ml
FC/FACS: 1-3 ug/10E6 cells
IHC-P: 10 ug/ml
WB: 0.5-1 ug/ml

Immunohistochemistry (IHC) of anti-GMNN antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-GMNN Polyclonal Antibody – Cat# MBS2401732

Western Blot (WB) of anti-GMNN antibody

Western blot of human recombinant Geminin fusion protein using Geminin Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 38 kD. Observed Size: 38 kD.
Rabbit Anti-GMNN Polyclonal Antibody, Western Blot (WB) – Cat# MBS2401732

Western Blot (WB) of anti-GMNN antibody

Western blot of cell lysates using Geminin Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 24 kD. Observed Size: 24 kD.
Rabbit Anti-GMNN Polyclonal Antibody, Western Blot (WB) – Cat# MBS2401732

Flow Cytometry (FC/FACS) of anti-GMNN antibody

Flow cytometry of HeLa cells using Geminin Antibody, pAb, Rabbit (shaded histogram) or with an isotype. Control antibody (open histogram), followed by R-PE conjugated anti-rabbit IgG.
Rabbit Anti-GMNN Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS2401732

Other Notes

Small volumes of anti-GMNN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GMNN purchase

MBS2401732 is an antibody targeting GMNN. Also known as Anti-Geminin Antibody (aa50-100). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GMNN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
geminin
NCBI Official Synonym Full Names
geminin, DNA replication inhibitor
NCBI Official Symbol
GMNN
NCBI Official Synonym Symbols
Gem; MGORS6
UniProt Protein Name
Geminin
Protein Family
UniProt Gene Name
GMNN
NCBI Protein Information
geminin

NCBI Summary for GMNN

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
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UniProt Comments for GMNN

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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