Tri-Iodothyronine, T3 (T3) ELISA Kit for Chicken

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of T3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS701857 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tri-iodothyronine, T3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing T3. The ELISA analytical biochemical technique of the MBS701857 kit is based on T3 antibody-T3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect T3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, T3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). The thyroid is a master regulator of hormones homeostasis. Triiodothyronine T3 is the functional protein, cleaved from free triiodothyronine T4 by TSH. Serum concentration os these proteins is tightly controlled. To quantitate human or chicken cell sample reagents in signal transduction protocols, we recommend our competitive format T3 ELISA kits. Both ELISA kits assay categories proteins ng ml detection based on our outstanding primary antibodies, secondary antibodies, T3 standard peptides for comparison and economical multi wells kit. Make your ELISA quantitation easy for T3 human or chicken. Compare our results to other Triiodothyronine T3 ELISA kits reagents. Our T3 ELISA quantitation will surpass expectation. View our website for an additional T3 antibody, recombinant proteins antibodies reagents peptides and robust antibodies secondary to complete your research. We also carry a full line of ELISA quantitation kit products against other Triiodothyronine T3 human related proteins. Order chicken or human ELISA kit today.