Apolipoprotein B (Including Ag (X) Antigen) (APOB) ELISA Kit for Rat

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-B100. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-B100. The competitive inhibition reaction is launched between with pre-coated Apo-B100 and Apo-B100 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-B100 in the samples. The color development is stopped and the intensity of the color is measured.

Small volumes of APOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS702832 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein B (including Ag (x) antigen) (APOB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOB. The ELISA analytical biochemical technique of the MBS702832 kit is based on APOB antibody-APOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Rat apolipoprotein B100, Apo-B100 ELISA Kit

Apolipoproteins are proteins that bind lipids (oil-soluble substances such as fat and cholesterol) to form lipoproteins. They transport the lipids through the lymphatic and circulatory systems.
In chylomicrons (VLDL, IDL, and LDL) apolipoprotein B is the primary apolipoprotein.

LDL (Low-density lipoprotein) is responsible for carrying fat molecules (lipids), including cholesterol, around the body (within the water outside cells) to all cells within all tissues. The functional role of apolipoprotein B within the LDL is somewhat unclear, though some vidence suggests it is an organizing protein and is required for the formation of these particles. Apolipoprotein B acts as a ligand for LDL receptors in various cells throughout the body
Mutations in gene APOB100 can also cause familial hypercholesterolemia and high levels of apolipoprotein B are related to heart disease.

The concentration of apolipoprotein B (via apob polyclonal antibody) in a test sample e.g. serum, plasma, can be derived from a standard curve. Cross reactivity is seen with rat. The details of reagents in this kit can be found in the product datasheet. This use of this assay kit is intended for research purposes.