Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA Kit for Rat

Name: Rat GOT1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS704087
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704087

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AST present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), ELISA Kit

Full Product Name

Rat Aspartate Aminotransferase, AST ELISA Kit

Product Gene Name

GOT1 elisa kit

Product Synonym Names

Rat Aspartate Aminotransferase (AST) ELISA kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1)

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Serum, plasma

Detection Range

3.12 mIU/ml-200 mIU/ml

Sensitivity

< 0.78 mIU/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of rat AST. No significant cross-reactivity or interference between rat AST and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

3,427 Da

3D Structure

ModBase 3D Structure for P12343

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GOT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT1 purchase

MBS704087 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GOT1. The ELISA analytical biochemical technique of the MBS704087 kit is based on GOT1 antibody-GOT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GOT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GOT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.

NCBI GI #

126302509

NCBI Accession #

P12343.2

UniProt Primary Accession #

P12343

NCBI Official Full Name

Aspartate aminotransferase, cytoplasmic

UniProt Protein Name

Aspartate aminotransferase, cytoplasmic

UniProt Synonym Protein Names

Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

Protein Family

Aspartate aminotransferase

UniProt Gene Name

GOT1

UniProt Synonym Gene Names

cAspAT; cCAT

UniProt Entry Name

AATC_RABIT

UniProt Comments for GOT1

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactor: Pyridoxal phosphate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GOT1 elisa kit

Reference Product	PubMed Publications
GOT2 elisa kit	>7 publications with GOT1 and GOT2
MDH1 elisa kit	>5 publications with GOT1 and MDH1
LDHB elisa kit	>3 publications with GOT1 and LDHB
MDH2 elisa kit	>2 publications with GOT1 and MDH2