Corticotropin Releasing Hormone (CRH) ELISA Kit for Mouse

Name: Mouse CRH ELISA Kit; Corticotropin Releasing Hormone; MyBioSource
Brand: MyBioSource
SKU: MBS704286
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704286

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRF. Standards or samples are added to the appropriate microtiter… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

corticotropin releasing hormone (CRH), ELISA Kit

Full Product Name

Mouse Corticotropin-releasing Factor, CRF ELISA Kit

Product Gene Name

CRH elisa kit

Product Synonym Names

Mouse Corticotropin-releasing Factor (CRF) ELISA Kit; CRF; corticotropin-releasing factor; corticotropin releasing hormone

Species Reactivity

Mouse

Assay Type

Competitive

Samples

Serum, plasma

Detection Range

2.5 pg/ml -40 pg/ml.

Sensitivity

The minimum detectable dose of mouse CRF is typically less than 0.675 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse CRF concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse CRF. No significant cross-reactivity or interference between mouse CRF and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

20,778 Da

3D Structure

ModBase 3D Structure for Q8CIT0

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRH purchase

MBS704286 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the corticotropin releasing hormone (CRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRH. The ELISA analytical biochemical technique of the MBS704286 kit is based on CRH antibody-CRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CRH. It may not necessarily be applicable to this product.

NCBI GI #

45429990

NCBI GeneID

12918

NCBI Accession #

NP_991338.1

UniProt Primary Accession #

Q8CIT0

UniProt Secondary Accession #

Q14AA2

UniProt Related Accession #

Q8CIT0

GenBank Nucleotide #

NM_205769.2

NCBI Official Full Name

corticoliberin preproprotein

NCBI Official Synonym Full Names

corticotropin releasing hormone

NCBI Official Symbol

Crh

NCBI Official Synonym Symbols

CRF; Gm1347

UniProt Protein Name

Corticoliberin

UniProt Synonym Protein Names

Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone

Protein Family

Corticoliberin

UniProt Gene Name

Crh

UniProt Synonym Gene Names

Gm1347; CRF

UniProt Entry Name

CRF_MOUSE

NCBI Protein Information

corticoliberin; corticotropin releasing factor; corticotropin-releasing factor; corticotropin-releasing hormone

NCBI Summary for CRH

This gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally. [provided by RefSeq, Aug 2015]

UniProt Comments for CRH

Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.

Research Articles on CRH

1. Impairment of CBP:CREB interaction reduces CRH expression in the hypothalamus on postnatal day nine, but does not disrupt steady-state CRH expression in adult mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CRH elisa kit

Reference Product	PubMed Publications
FLNA elisa kit	>12 publications with CRH and FLNA

Pathways associated with CRH elisa kit

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Long-term Depression Pathway antibodies	Long-term Depression Pathway Diagram
Long-term Depression Pathway antibodies	Long-term Depression Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram
Serotonin And Anxiety Pathway antibodies	Serotonin And Anxiety Pathway Diagram

Diseases associated with CRH elisa kit

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>663 publications with CRH and Mental Disorders
Nervous System Diseases Antibodies	>641 publications with CRH and Nervous System Diseases
Brain Diseases Antibodies	>504 publications with CRH and Brain Diseases
Cushing Syndrome Antibodies	>368 publications with CRH and Cushing Syndrome
Depressive Disorder Antibodies	>325 publications with CRH and Depressive Disorder
Inflammation Antibodies	>246 publications with CRH and Inflammation
Anxiety Disorders Antibodies	>226 publications with CRH and Anxiety Disorders
Necrosis Antibodies	>133 publications with CRH and Necrosis
Hypertension Antibodies	>118 publications with CRH and Hypertension
Pain Antibodies	>99 publications with CRH and Pain

Organs/Tissues associated with CRH elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with CRH and Bone
Connective Tissue Antibodies	>4 publications with CRH and Connective Tissue
Heart Antibodies	>3 publications with CRH and Heart
Muscle Antibodies	>3 publications with CRH and Muscle
Brain Antibodies	>3 publications with CRH and Brain
Vascular Antibodies	>2 publications with CRH and Vascular
Embryonic Tissue Antibodies	>2 publications with CRH and Embryonic Tissue
Ear Antibodies	>1 publications with CRH and Ear
Kidney Antibodies	>1 publications with CRH and Kidney
Umbilical Cord Antibodies	>1 publications with CRH and Umbilical Cord