Clara Cell Protein, CC16 (CC16) ELISA Kit for Mouse

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CC16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CC16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CC16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CC16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of CC16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS704464 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Clara cell protein, CC16, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CC16. The ELISA analytical biochemical technique of the MBS704464 kit is based on CC16 antibody-CC16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CC16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CC16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mouse Clara cell types are an important model of pulmonary / lung endothelium in Respir Med studies. Frequently used for small molecule drug or toxin screening, changes in cell phenotype are readily quantitated by CC16 ELISA assay. This surface marker is similar to human serum plasma protein, so a precision mouse specific protein CC16 - Clara cell secretory protein - with tight dynamic range, excellent intra assay variability and ng ml detection is worth a view. Here at MyBiosource, we have samples concentration tested already, checked primary Clara cell protein 16 CC16 antibody and secondary polyclonal antibody so your molecular biology or cell culture experiments are reliable and speedy from start to finish. This ELISA kit, protein CC16 ELISA kit rat and CC16 human ELISA kits will get you publication quality results from valuable sample dilutions easily. View our on line catalog for assay precision data and pricing.