Chymase 1, Mast Cell (CMA1) ELISA Kit for Human

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mast cell chymase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mast cell chymase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mast cell chymase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mast cell chymase bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of CMA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS704628 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chymase 1, mast cell (CMA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CMA1. The ELISA analytical biochemical technique of the MBS704628 kit is based on CMA1 antibody-CMA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CMA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CMA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mast Cell Chymase (mast cell protease 1, cma, skeletal muscle protease, skin chymotryptic proteinase, mast cell serine proteinase, skeletal muscle protease) is protein stored and secreted by mast cells. It belongs to a family of serine proteases found primarily in mast cells, though also present in basophil granulocytes. Mast cell chymase is a destructive enzme and shows broad peptidolytic activity. It is quickly inhibited after release, do reduce damage. Mast cells upon challenge with parasites and parasite antigens promote an inflammatory response be releasing mast cell chymase.
A role in hypertension, asthma, atherosclerosisas well as a broader role in inflammation have been proposed.
The concentration of mast cell chymase in the test sample (plasma, tissue, biological fluids etc) can be derived from a standard curve. The details of the detection reagent in this ELISA kits can be found in the product datasheet. Cross species reactivity has been demonstrated to mouse and human sequences.