Cystatin B (Stefin B) (CSTB) ELISA Kit for Human

Name: Human CSTB ELISA Kit; Cystatin B (Stefin B); MyBioSource
Brand: MyBioSource
SKU: MBS705920
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS705920

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cystatin-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cystatin-B present is bound by… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

cystatin B (stefin B) (CSTB), ELISA Kit

Full Product Name

Human Cystatin-B, CSTB/CST6/STFB ELISA Kit

Product Gene Name

CSTB elisa kit

Product Synonym Names

Human Cystatin-B (CSTB/CST6/STFB) ELISA kit; CST6; EPM1; PME; STFB; CPI-B; cystatin B; liver thiol proteinase inhibitor; stefin B; Human Cystatin-B (CSTB/CST6/STFB)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, cell culture supernates, urine, saliva, tissue homogenates, Cell lysates

Detection Range

0.313 ng/ml-20 ng/ml

Sensitivity

0.233 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human CSTB. No significant cross-reactivity or interference between Human CSTB and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

11,140 Da

OMIM

601145

3D Structure

ModBase 3D Structure for P04080

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

others

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CSTB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSTB purchase

MBS705920 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cystatin B (stefin B) (CSTB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSTB. The ELISA analytical biochemical technique of the MBS705920 kit is based on CSTB antibody-CSTB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSTB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSTB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.

NCBI GI #

4503117

NCBI GeneID

1476

NCBI Accession #

NP_000091.1

UniProt Primary Accession #

P04080

UniProt Related Accession #

P04080

GenBank Nucleotide #

NM_000100.3

NCBI Official Full Name

cystatin-B

NCBI Official Synonym Full Names

cystatin B (stefin B)

NCBI Official Symbol

CSTB

NCBI Official Synonym Symbols

PME; ULD; CST6; EPM1; STFB; EPM1A

UniProt Protein Name

Cystatin-B

UniProt Synonym Protein Names

CPI-B; Liver thiol proteinase inhibitor; Stefin-B

Protein Family

Cystatin

UniProt Gene Name

CSTB

UniProt Synonym Gene Names

CST6; STFB

UniProt Entry Name

CYTB_HUMAN

NCBI Protein Information

cystatin-B; CPI-B; liver thiol proteinase inhibitor

NCBI Summary for CSTB

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008]

UniProt Comments for CSTB

Function: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Research Articles on CSTB

1. High levels of bioactive recombinant stefins A and B can be produced by fermentation in P. pastoris.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CSTB elisa kit

Reference Product	PubMed Publications
CST3 elisa kit	>25 publications with CSTB and CST3
CTSL1 elisa kit	>19 publications with CSTB and CTSL1
CTSH elisa kit	>7 publications with CSTB and CTSH
CTSB elisa kit	>5 publications with CSTB and CTSB
CST4 elisa kit	>2 publications with CSTB and CST4
STAT1 elisa kit	>2 publications with CSTB and STAT1

Diseases associated with CSTB elisa kit

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>88 publications with CSTB and Brain Diseases
Unverricht-Lundborg Syndrome Antibodies	>43 publications with CSTB and Unverricht-Lundborg Syndrome
Disease Models, Animal Antibodies	>20 publications with CSTB and Disease Models, Animal
Adenocarcinoma Antibodies	>10 publications with CSTB and Adenocarcinoma
Recurrence Antibodies	>8 publications with CSTB and Recurrence
Lung Diseases Antibodies	>7 publications with CSTB and Lung Diseases
Atrophy Antibodies	>6 publications with CSTB and Atrophy
Lung Neoplasms Antibodies	>6 publications with CSTB and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>6 publications with CSTB and Neoplasm Invasiveness
Liver Neoplasms Antibodies	>3 publications with CSTB and Liver Neoplasms

Organs/Tissues associated with CSTB elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>26 publications with CSTB and Blood
Brain Antibodies	>25 publications with CSTB and Brain
Liver Antibodies	>12 publications with CSTB and Liver
Skin Antibodies	>7 publications with CSTB and Skin
Lung Antibodies	>7 publications with CSTB and Lung
Thymus Antibodies	>5 publications with CSTB and Thymus
Embryonic Tissue Antibodies	>5 publications with CSTB and Embryonic Tissue
Muscle Antibodies	>4 publications with CSTB and Muscle
Kidney Antibodies	>4 publications with CSTB and Kidney
Mouth Antibodies	>4 publications with CSTB and Mouth