DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 (DDX1) Antibody – Rabbit Polyclonal

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]

DDX1: Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF- kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. Belongs to the DEAD box helicase family. DDX1 subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: nucleoplasm; membrane; stress granule; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; nuclease activity; DNA binding; DNA/RNA helicase activity; double-stranded RNA binding; exonuclease activity; transcription cofactor activity; ATP-dependent helicase activity; chromatin binding; RNA helicase activity; ATP binding; poly(A) binding

Biological Process: tRNA splicing; response to exogenous dsRNA; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to virus; double-strand break repair; multicellular organismal development; spliceosome assembly; DNA duplex unwinding; regulation of translational initiation