Gamma-Aminobutyric Acid Receptor-Associated Protein (GABARAP) Antibody – Rabbit Polyclonal

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.

Protein type: Vesicle; Adaptor/scaffold; Microtubule-binding; Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: actin cytoskeleton; autophagic vacuole; axoneme; cytoplasmic vesicle; cytosol; Golgi membrane; lysosome; microtubule; microtubule associated complex; perinuclear region of cytoplasm; plasma membrane; smooth endoplasmic reticulum

Molecular Function: beta-tubulin binding; GABA receptor binding; microtubule binding; protein binding; ubiquitin protein ligase binding

Biological Process: induction of apoptosis via death domain receptors; macroautophagy; microtubule cytoskeleton organization and biogenesis; protein targeting; synaptic transmission