Human PACAPR1 ELISA Kit – Cat# MBS720644

Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1 (PACAPR1) ELISA Kit for Human

Name: Human PACAPR1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS720644
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS720644

Principle of the Assay: PACAPR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PACAPR antibody and an PACAPR-HRP conjugate. The assay sample and buffer are incubated together with PACAPR-HRP conjugate in pre-coated… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1 (PACAPR1), ELISA Kit

Full Product Name

Human Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1 ELISA Kit

Product Gene Name

PACAPR1 elisa kit

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

0.5-10ng/mL

Sensitivity

0.1ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

46,957 Da

OMIM

102981

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This PACAPR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PACAPR. This ELISA kit for research use only, not for therapeutic applications!

Product Categories/Family for PACAPR1 elisa kit

Signal Transduction

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PACAPR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PACAPR1 purchase

MBS720644 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1 (PACAPR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PACAPR1. The ELISA analytical biochemical technique of the MBS720644 kit is based on PACAPR1 antibody-PACAPR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PACAPR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PACAPR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PACAPR1. It may not necessarily be applicable to this product.

NCBI GI #

313851018

NCBI GeneID

117

NCBI Accession #

NP_001186564.1

UniProt Secondary Accession #

Q17S10; A8K1Y1; B7ZLA7; B8ZZK3

UniProt Related Accession #

P41586

GenBank Nucleotide #

NM_001199635.1

NCBI Official Full Name

pituitary adenylate cyclase-activating polypeptide type I receptor isoform 1

NCBI Official Synonym Full Names

adenylate cyclase activating polypeptide 1 (pituitary) receptor type I

NCBI Official Symbol

ADCYAP1R1

NCBI Official Synonym Symbols

PAC1; PAC1R; PACAPR; PACAPRI

UniProt Protein Name

Pituitary adenylate cyclase-activating polypeptide type I receptor

UniProt Gene Name

ADCYAP1R1

UniProt Synonym Gene Names

PACAP type I receptor; PACAP-R-1; PACAP-R1

UniProt Entry Name

PACR_HUMAN

NCBI Protein Information

pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP-R1; PACAP receptor 1; PACAP type I receptor; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop

NCBI Summary for PACAPR1

This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]

UniProt Comments for PACAPR1

ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 2; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; endosome; receptor complex

Molecular Function: ADP-ribosylation factor binding; neuropeptide binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding

Biological Process: response to drug; development of primary female sexual characteristics; nerve growth factor receptor signaling pathway; adenylate cyclase activation; response to estradiol stimulus; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; phospholipase C activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; transmembrane receptor protein tyrosine kinase signaling pathway

Research Articles on PACAPR1

1. PAC1/PACAP receptor endocytosis contributes to ERK acktivation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PACAPR1 elisa kit

Reference Product	PubMed Publications
ADCYAP1 elisa kit	>104 publications with PACAPR1 and ADCYAP1
CRH elisa kit	>2 publications with PACAPR1 and CRH
GHRH elisa kit	>2 publications with PACAPR1 and GHRH
POMC elisa kit	>1 publications with PACAPR1 and POMC

Pathways associated with PACAPR1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of TRKA Receptors Pathway antibodies	Activation Of TRKA Receptors Pathway Diagram
Circadian Entrainment Pathway antibodies	Circadian Entrainment Pathway Diagram
Circadian Entrainment Pathway antibodies	Circadian Entrainment Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class B Secretin-like Pathway antibodies	GPCRs, Class B Secretin-like Pathway Diagram
Glucagon-type Ligand Receptors Pathway antibodies	Glucagon-type Ligand Receptors Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram

Diseases associated with PACAPR1 elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>6 publications with PACAPR1 and Breast Neoplasms
Inflammation Antibodies	>5 publications with PACAPR1 and Inflammation
Disease Models, Animal Antibodies	>2 publications with PACAPR1 and Disease Models, Animal
Learning Disorders Antibodies	>2 publications with PACAPR1 and Learning Disorders
Hyperplasia Antibodies	>2 publications with PACAPR1 and Hyperplasia
Brain Injuries Antibodies	>2 publications with PACAPR1 and Brain Injuries
Liver Neoplasms, Experimental Antibodies	>2 publications with PACAPR1 and Liver Neoplasms, Experimental
Liver Diseases Antibodies	>2 publications with PACAPR1 and Liver Diseases
Hypertrophy Antibodies	>2 publications with PACAPR1 and Hypertrophy
Necrosis Antibodies	>2 publications with PACAPR1 and Necrosis

Organs/Tissues associated with PACAPR1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>76 publications with PACAPR1 and Brain
Nerve Antibodies	>28 publications with PACAPR1 and Nerve
Pituitary Gland Antibodies	>26 publications with PACAPR1 and Pituitary Gland
Blood Antibodies	>15 publications with PACAPR1 and Blood
Ganglia Antibodies	>14 publications with PACAPR1 and Ganglia
Muscle Antibodies	>12 publications with PACAPR1 and Muscle
Adrenal Gland Antibodies	>11 publications with PACAPR1 and Adrenal Gland
Lung Antibodies	>10 publications with PACAPR1 and Lung
Kidney Antibodies	>6 publications with PACAPR1 and Kidney
Intestine Antibodies	>5 publications with PACAPR1 and Intestine