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Caspase 1 (CASP1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601609

Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding… More details

$325 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Caspase 1 (CASP1), Polyclonal Antibody
Full Product Name
Caspase 1 (Phospho-Ser376) Antibody
Product Gene Name
anti-CASP1 antibody
Product Synonym Names
CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Concentration
1mg/ml (lot specific)
Specificity
Caspase 1 (Phospho-Ser376) antibody detects endogenous levels of total Caspase 1 (Phospho-Ser376)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Caspase 1 (Ser376) peptide (MBS9617141) is used for blocking the activity of Caspase 1 (Ser376) antibody (MBS9601609)
Molecular Weight
Observed: 45 kDa
Predicted: 46 kDa
3D Structure
ModBase 3D Structure for P29466
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Caspase 1 (Phospho-Ser376)
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Predicted Cross Reactivity
Pig, Bovine, Rabbit
Similarity
Pig (100%), Bovine (88%), Rabbit (88%)
Conjugation
Unconjugated
Epitope
Phospho Ser376
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CASP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CASP1 antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-CASP1 antibody

Western blot analysis of Caspase 1 (Phospho-Ser376) expression in 293 cells.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-CASP1 Polyclonal Antibody – Cat# MBS9601609

Immunofluorescene (IF) of anti-CASP1 antibody

MBS9601609 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-CASP1 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9601609

Other Notes

Small volumes of anti-CASP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASP1 purchase

MBS9601609 is an antibody targeting CASP1. Also known as Caspase 1 (Phospho-Ser376) Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CASP1. It may not necessarily be applicable to this product.

NCBI GI #
4502573
NCBI GeneID
834
NCBI Accession #
NP_001214.1
UniProt Primary Accession #
P29466
UniProt Secondary Accession #
Q53EY6; Q6DMQ1; Q6GSS3; Q6PI75; Q9UCN3; B5MDZ1
UniProt Related Accession #
P29466
GenBank Nucleotide #
NM_001223.4
NCBI Official Full Name
caspase-1 isoform beta
NCBI Official Synonym Full Names
caspase 1
NCBI Official Symbol
CASP1
NCBI Official Synonym Symbols
ICE; P45; IL1BC
UniProt Protein Name
Caspase-1
UniProt Synonym Protein Names
Interleukin-1 beta convertase; IL-1BC
Protein Family
Caspase
UniProt Gene Name
CASP1
UniProt Synonym Gene Names
IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
NCBI Protein Information
caspase-1

NCBI Summary for CASP1

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
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UniProt Comments for CASP1

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
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Research Articles on CASP1

1. The increased expression of NLRP3 and caspase-1 in fetal membrane and placental tissues may be associated with the development of premature rupture of membrane.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CASP1 antibody

 Reference Product  PubMed Publications
 IL18 antibody  >437 publications with CASP1 and IL18
 PYCARD antibody  >155 publications with CASP1 and PYCARD
 NLRC4 antibody  >121 publications with CASP1 and NLRC4
 BCL2L1 antibody  >84 publications with CASP1 and BCL2L1
 NLRP1 antibody  >71 publications with CASP1 and NLRP1
 NOD2 antibody  >51 publications with CASP1 and NOD2
 NOD1 antibody  >31 publications with CASP1 and NOD1
 RIPK2 antibody  >30 publications with CASP1 and RIPK2
 IL1B antibody  >28 publications with CASP1 and IL1B
 BID antibody  >23 publications with CASP1 and BID

Pathways associated with anti-CASP1 antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 IFN-gamma Pathway antibodies  IFN-gamma Pathway Diagram

Diseases associated with anti-CASP1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >936 publications with CASP1 and Inflammation
 Necrosis Antibodies  >538 publications with CASP1 and Necrosis
 Neoplasms Antibodies  >396 publications with CASP1 and Neoplasms
 Nervous System Diseases Antibodies  >321 publications with CASP1 and Nervous System Diseases
 Brain Diseases Antibodies  >239 publications with CASP1 and Brain Diseases
 Drug Toxicity Antibodies  >198 publications with CASP1 and Drug Toxicity
 Liver Diseases Antibodies  >92 publications with CASP1 and Liver Diseases
 Lung Diseases Antibodies  >89 publications with CASP1 and Lung Diseases
 Hypersensitivity Antibodies  >67 publications with CASP1 and Hypersensitivity
 Heart Diseases Antibodies  >63 publications with CASP1 and Heart Diseases

Organs/Tissues associated with anti-CASP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >855 publications with CASP1 and Blood
 Brain Antibodies  >365 publications with CASP1 and Brain
 Bone Antibodies  >166 publications with CASP1 and Bone
 Lung Antibodies  >162 publications with CASP1 and Lung
 Nerve Antibodies  >150 publications with CASP1 and Nerve
 Vascular Antibodies  >136 publications with CASP1 and Vascular
 Skin Antibodies  >129 publications with CASP1 and Skin
 Kidney Antibodies  >113 publications with CASP1 and Kidney
 Heart Antibodies  >111 publications with CASP1 and Heart
 Muscle Antibodies  >94 publications with CASP1 and Muscle
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