Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit for Human

Intended Uses: This ICAM-1 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ICAM-1in the sample, this ICAM-1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ICAM-1concentration. The concentration of ICAM-1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This ICAM-1enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for ICAM-1. Standards or samples are then added to the microtiter plate wells and ICAM-1if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ICAM-1present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ICAM-1are added to each well to "sandwich" the ICAM-1immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain ICAM-1and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Small volumes of ICAM-1/CD54 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS723364 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the intercellular adhesion molecule 1 (ICAM-1/CD54) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ICAM-1/CD54. The ELISA analytical biochemical technique of the MBS723364 kit is based on ICAM-1/CD54 antibody-ICAM-1/CD54 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ICAM-1/CD54 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ICAM-1/CD54. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).