Alpha-Feto (AFP) ELISA Kit for Mouse

Principle of the Assay: AFP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AFP antibody and an AFP-HRP conjugate. The assay sample and buffer are incubated together with AFP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFP concentration since AFP from samples and AFP-HRP conjugate compete for the anti-AFP antibody binding site. Since the number of sites is limited, as more sites are occupied by AFP from the sample, fewer sites are left to bind AFP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFP concentration in each sample is interpolated from this standard curve.

Small volumes of AFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS730245 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-fetoprotein (AFP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AFP. The ELISA analytical biochemical technique of the MBS730245 kit is based on AFP antibody-AFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AFP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Unlike fetoprotein AFP human, rodent alpha fetoprotein AFP binds and transports estrogen across maternal placentas and effects sexual development of fetal mouse. Thus, quantitation of this key plasma protein in research settings is of great interest both in reproductive and cancer antigen biology. We are able to provide a highly sensitive competitive format fetoprotein AFP ELISA perfect for your assay requirements. Both our mouse specific and human alpha fetoprotein ELISA kits are shipped complete after your order. All necessary reagents for successful results from serum, cell culture media or cell lysates from this product are included - AFP monoclonal antibody clone, secondary polyclonal antibody for detection with ALEXA 174 fluor or AMP. All our kits reagents are molecular biology grade, making comparative studies with FISH probes or q PCR possible. This ELISA kit, like all of our products, either standard or custom antibodies, are fully satisfaction guaranteed. View online today.