Erythropoietin (EPO ab) ELISA Kit for Human

Name: Human EPO ab ELISA Kit; Erythropoietin; MyBioSource
Brand: MyBioSource
SKU: MBS733594
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS733594

Principle of the Assay: EPOAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Erythropoietin antigen and an EPOAb-HRP conjugate. The assay sample and buffer are incubated together with EPOAb-HRP conjugate in pre-coated plate for one… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Erythropoietin antibody (EPO ab), ELISA Kit

Full Product Name

Human Erythropoietin antibody ELISA Kit

Product Gene Name

EPO ab elisa kit

Species Reactivity

Human

Assay Type

Quantitative Competitive

Samples

Erum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of EPOAb. No significant cross-reactivity or interference between EPOAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPOAb and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

21,307 Da

OMIM

gene 612623

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for EPO ab elisa kit

Immunology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of EPO ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPO ab purchase

MBS733594 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Erythropoietin antibody (EPO ab) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EPO ab. The ELISA analytical biochemical technique of the MBS733594 kit is based on EPO ab antibody-EPO ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPO ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPO ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EPO ab. It may not necessarily be applicable to this product.

NCBI GI #

62240997

NCBI GeneID

2056

NCBI Accession #

NP_000790.2

UniProt Secondary Accession #

Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0

UniProt Related Accession #

P01588

GenBank Nucleotide #

NM_000799.2

NCBI Official Full Name

erythropoietin

NCBI Official Synonym Full Names

erythropoietin

NCBI Official Symbol

EPO

NCBI Official Synonym Symbols

EP; MVCD2

UniProt Protein Name

Erythropoietin

UniProt Synonym Protein Names

INN: Epoetin

UniProt Gene Name

EPO

UniProt Entry Name

EPO_HUMAN

NCBI Protein Information

erythropoietin; epoetin

NCBI Summary for EPO ab

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

UniProt Comments for EPO ab

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; apoptosis; blood circulation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; response to axon injury; positive regulation of neuron differentiation; response to electrical stimulus; positive regulation of DNA replication; embryo implantation; aging

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

Research Articles on EPO ab

1. The progressive increase of EPO and EpoR mRNA can already be observed during the fibrotic-cirrhotic development with a peak of expression rising at tumor formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EPO ab elisa kit

Reference Product	PubMed Publications
EPOR elisa kit	>710 publications with EPO ab and EPOR
JAK2 elisa kit	>320 publications with EPO ab and JAK2
TNF elisa kit	>277 publications with EPO ab and TNF
HIF1A elisa kit	>250 publications with EPO ab and HIF1A
STAT3 elisa kit	>80 publications with EPO ab and STAT3
VEGFA elisa kit	>41 publications with EPO ab and VEGFA
GATA2 elisa kit	>28 publications with EPO ab and GATA2
TFRC elisa kit	>4 publications with EPO ab and TFRC

Pathways associated with EPO ab elisa kit

Products by Pathway	Pathway Diagram
Cellular Response To Hypoxia Pathway antibodies	Cellular Response To Hypoxia Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
EPO Receptor Signaling Pathway antibodies	EPO Receptor Signaling Pathway Diagram
EPO Signaling Pathway antibodies	EPO Signaling Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
HIF-2-alpha Transcription Factor Network Pathway antibodies	HIF-2-alpha Transcription Factor Network Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with EPO ab elisa kit

Disease Name	Pubmed Publications
Anemia Antibodies	>2757 publications with EPO ab and Anemia
Kidney Diseases Antibodies	>1353 publications with EPO ab and Kidney Diseases
Renal Insufficiency Antibodies	>995 publications with EPO ab and Renal Insufficiency
Kidney Failure, Chronic Antibodies	>918 publications with EPO ab and Kidney Failure, Chronic
Vascular Diseases Antibodies	>866 publications with EPO ab and Vascular Diseases
Nervous System Diseases Antibodies	>666 publications with EPO ab and Nervous System Diseases
Inflammation Antibodies	>573 publications with EPO ab and Inflammation
Central Nervous System Diseases Antibodies	>499 publications with EPO ab and Central Nervous System Diseases
Heart Diseases Antibodies	>361 publications with EPO ab and Heart Diseases
Necrosis Antibodies	>359 publications with EPO ab and Necrosis

Organs/Tissues associated with EPO ab elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>785 publications with EPO ab and Brain
Eye Antibodies	>154 publications with EPO ab and Eye
Testis Antibodies	>33 publications with EPO ab and Testis
Prostate Antibodies	>32 publications with EPO ab and Prostate