Prostaglandin D2 Receptor (PTGDR) ELISA Kit for Human

Name: Human PTGDR ELISA Kit; Prostaglandin D2 Receptor; MyBioSource
Brand: MyBioSource
SKU: MBS760674
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760674

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Prostaglandin D2 receptor (PTGDR), ELISA Kit

Full Product Name

Human Prostaglandin D2 receptor ELISA Kit

Product Gene Name

PTGDR elisa kit

Product Synonym Names

PTGDR/Prostaglandin D2 receptor/PGD receptor/PGD2 receptor/Prostanoid DP receptor

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of PTGDR. No significant cross-reactivity or interference between PTGDR and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay: CV<8%

Molecular Weight

43,268 Da

OMIM

604837

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PTGDR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTGDR purchase

MBS760674 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prostaglandin D2 receptor (PTGDR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTGDR. The ELISA analytical biochemical technique of the MBS760674 kit is based on PTGDR antibody-PTGDR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTGDR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTGDR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PTGDR. It may not necessarily be applicable to this product.

NCBI GI #

40846396

NCBI GeneID

11251

NCBI Accession #

AAR92484.1

UniProt Secondary Accession #

O94765; Q4QRI6

UniProt Related Accession #

Q9Y5Y4

NCBI Official Full Name

prostaglandin D2 receptor

NCBI Official Synonym Full Names

prostaglandin D2 receptor 2

NCBI Official Symbol

PTGDR2

NCBI Official Synonym Symbols

DP2; DL1R; CD294; CRTH2; GPR44

UniProt Protein Name

Prostaglandin D2 receptor 2

UniProt Synonym Protein Names

Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD_antigen: CD294

Protein Family

Prostaglandin D2 receptor

UniProt Gene Name

PTGDR2

UniProt Synonym Gene Names

CRTH2; DL1R; GPR44

UniProt Entry Name

PD2R2_HUMAN

NCBI Protein Information

prostaglandin D2 receptor 2

NCBI Summary for PTGDR

This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]

UniProt Comments for PTGDR

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q12-q13.3

Cellular Component: integral to plasma membrane; intracellular; neuron projection; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; prostaglandin D receptor activity; prostaglandin F receptor activity; prostaglandin J receptor activity

Biological Process: calcium-mediated signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; immune response; neuropeptide signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; synaptic transmission

Research Articles on PTGDR

1. Data indicate that in ulcerative colitis, DP2 receptor showed higher expression in neutrophils but lower in monocytes, and CRTH2 receptor was decreased in eosinophils, NK, and CD3(+) T cells but not in monocytes and CD3(+)/CD4(+) T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTGDR elisa kit

Reference Product	PubMed Publications
CCR3 elisa kit	>1 publications with PTGDR and CCR3

Pathways associated with PTGDR elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Eicosanoid Ligand-binding Receptors Pathway antibodies	Eicosanoid Ligand-binding Receptors Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Prostanoid Ligand Receptors Pathway antibodies	Prostanoid Ligand Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram