Human HIST1H4A ELISA Kit – Cat# MBS761642

Histone H4 (HIST1H4A) ELISA Kit for Human

Name: Human HIST1H4A ELISA Kit; Histone H4; MyBioSource
Brand: MyBioSource
SKU: MBS761642
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761642

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Histone H4 (HIST1H4A), ELISA Kit

Full Product Name

Human Histone H4 ELISA Kit

Product Gene Name

HIST1H4A elisa kit

Product Synonym Names

HIST1H4A/Histone H4/H4/A/H4FA/H4F2/histone cluster 4; H4

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of HIST1H4A. No significant cross-reactivity or interference between HIST1H4A and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

CV<10%

Intra-assay Precision

CV<8%

Molecular Weight

11,367 Da

OMIM

142750

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of HIST1H4A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIST1H4A purchase

MBS761642 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone H4 (HIST1H4A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIST1H4A. The ELISA analytical biochemical technique of the MBS761642 kit is based on HIST1H4A antibody-HIST1H4A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIST1H4A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIST1H4A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H4A. It may not necessarily be applicable to this product.

NCBI GI #

28173560

NCBI GeneID

121504

NCBI Accession #

NP_778224.1

UniProt Secondary Accession #

P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0

UniProt Related Accession #

P62805

GenBank Nucleotide #

NM_175054.2

NCBI Official Full Name

histone H4

NCBI Official Synonym Full Names

histone cluster 4, H4

NCBI Official Symbol

HIST4H4

NCBI Official Synonym Symbols

H4/p

UniProt Protein Name

Histone H4

Protein Family

Histone H4-like protein

UniProt Gene Name

HIST1H4A

UniProt Synonym Gene Names

H4/A; H4FA

UniProt Entry Name

H4_HUMAN

NCBI Protein Information

histone H4

NCBI Summary for HIST1H4A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015]

UniProt Comments for HIST1H4A

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Articles on HIST1H4A

1. H4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HIST1H4A elisa kit

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Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway antibodies	Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway Diagram
Activation Of HOX Genes During Differentiation Pathway antibodies	Activation Of HOX Genes During Differentiation Pathway Diagram
Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies	Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amyloid Fiber Formation Pathway antibodies	Amyloid Fiber Formation Pathway Diagram
B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies	B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram

Diseases associated with HIST1H4A elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>1 publications with HIST1H4A and Necrosis