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Homeobox Protein OTX2 (OTX2) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761722

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OTX2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OTX2 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Homeobox protein OTX2, ELISA Kit
Full Product Name
Human Homeobox protein OTX2 ELISA Kit
Product Gene Name
OTX2 elisa kit
Product Synonym Names
OTX2/homeobox protein OTX2/MCOPS5/orthodenticle homeobox 2/Orthodenticle homolog 2/orthodenticle homolog 2 (Drosophila)
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
32,406 Da
OMIM
600037
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of OTX2 elisa kit

Human OTX2 ELISA Kit – Cat# MBS761722

Typical Standard Curve of OTX2 elisa kit

Human OTX2 ELISA Kit, Typical Standard Curve – Cat# MBS761722

Other Notes

Small volumes of OTX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OTX2 purchase

MBS761722 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Homeobox protein OTX2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OTX2. The ELISA analytical biochemical technique of the MBS761722 kit is based on OTX2 antibody-OTX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OTX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OTX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OTX2. It may not necessarily be applicable to this product.

NCBI GI #
395455075
NCBI GeneID
5015
NCBI Accession #
NP_001257454.1
UniProt Secondary Accession #
Q6GTV3; Q9HAW3; Q9P2R1; B2RAN5
UniProt Related Accession #
P32243
GenBank Nucleotide #
NM_001270525.1
NCBI Official Full Name
homeobox protein OTX2 isoform a
NCBI Official Synonym Full Names
orthodenticle homeobox 2
NCBI Official Symbol
OTX2
NCBI Official Synonym Symbols
CPHD6; MCOPS5
UniProt Protein Name
Homeobox protein OTX2
UniProt Synonym Protein Names
Orthodenticle homolog 2
Protein Family
Homeobox protein
UniProt Gene Name
OTX2
UniProt Entry Name
OTX2_HUMAN
NCBI Protein Information
homeobox protein OTX2

NCBI Summary for OTX2

This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]
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UniProt Comments for OTX2

OTX2: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAC-3'. Expressed in brain. Belongs to the paired homeobox family. Bicoid subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; growth cone; nucleus; protein complex

Molecular Function: eukaryotic initiation factor 4E binding; protein binding; protein heterodimerization activity

Biological Process: axon guidance; cell fate specification; diencephalon morphogenesis; dorsal/ventral pattern formation; endoderm development; eye photoreceptor cell fate commitment; forebrain development; inner ear morphogenesis; metencephalon development; midbrain development; neuron fate determination; positive regulation of embryonic development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of fibroblast growth factor receptor signaling pathway; regulation of smoothened signaling pathway; somite rostral/caudal axis specification; transcription from RNA polymerase II promoter

Disease: Microphthalmia, Syndromic 5; Pituitary Hormone Deficiency, Combined, 6
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Research Articles on OTX2

1. This epigenome-wide DNA methylation analysis in postmortem hippocampus and prefrontal cortex specimens confirmed OTX2 DNA methylation profiles in major depression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OTX2 elisa kit

 Reference Product  PubMed Publications
 FGF8 elisa kit  >97 publications with OTX2 and FGF8
 WNT1 elisa kit  >45 publications with OTX2 and WNT1
 MITF elisa kit  >20 publications with OTX2 and MITF
 FOXA2 elisa kit  >20 publications with OTX2 and FOXA2
 LMX1B elisa kit  >10 publications with OTX2 and LMX1B
 FGF2 elisa kit  >9 publications with OTX2 and FGF2
 NKX2-2 elisa kit  >7 publications with OTX2 and NKX2-2
 TLE4 elisa kit  >4 publications with OTX2 and TLE4
 ASCL1 elisa kit  >3 publications with OTX2 and ASCL1
 YY1 elisa kit  >1 publications with OTX2 and YY1

Pathways associated with OTX2 elisa kit

 Products by Pathway  Pathway Diagram
 TP53 Network Pathway antibodies  TP53 Network Pathway Diagram

Diseases associated with OTX2 elisa kit

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >74 publications with OTX2 and Congenital Abnormalities
 Brain Diseases Antibodies  >52 publications with OTX2 and Brain Diseases
 Medulloblastoma Antibodies  >28 publications with OTX2 and Medulloblastoma
 Embryo Loss Antibodies  >25 publications with OTX2 and Embryo Loss
 Abnormalities, Multiple Antibodies  >24 publications with OTX2 and Abnormalities, Multiple
 Craniofacial Abnormalities Antibodies  >19 publications with OTX2 and Craniofacial Abnormalities
 Nervous System Malformations Antibodies  >14 publications with OTX2 and Nervous System Malformations
 Disease Models, Animal Antibodies  >10 publications with OTX2 and Disease Models, Animal
 Neural Tube Defects Antibodies  >9 publications with OTX2 and Neural Tube Defects
 Jaw Abnormalities Antibodies  >6 publications with OTX2 and Jaw Abnormalities

Organs/Tissues associated with OTX2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >365 publications with OTX2 and Brain
 Eye Antibodies  >208 publications with OTX2 and Eye
 Embryonic Tissue Antibodies  >205 publications with OTX2 and Embryonic Tissue
 Pineal Gland Antibodies  >11 publications with OTX2 and Pineal Gland
 Uncharacterized Tissue Antibodies  >1 publications with OTX2 and Uncharacterized Tissue
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