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Adenosine A2b Receptor (ADORA2b) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762150

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat ADORA2b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ADORA2b antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Adenosine A2b Receptor (ADORA2b), ELISA Kit
Full Product Name
Rat Adenosine A2b Receptor ELISA Kit
Product Gene Name
ADORA2b elisa kit
Product Synonym Names
ADORA2b
Species Reactivity
Rat
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
36,333 Da
OMIM
600446
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of ADORA2b elisa kit

Rat ADORA2b ELISA Kit – Cat# MBS762150

Typical Standard Curve of ADORA2b elisa kit

Rat ADORA2b ELISA Kit, Typical Standard Curve – Cat# MBS762150

Other Notes

Small volumes of ADORA2b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADORA2b purchase

MBS762150 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine A2b Receptor (ADORA2b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADORA2b. The ELISA analytical biochemical technique of the MBS762150 kit is based on ADORA2b antibody-ADORA2b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADORA2b. It may not necessarily be applicable to this product.

NCBI GI #
178150
NCBI GeneID
136
NCBI Accession #
AAA51598.1
UniProt Related Accession #
P29275
NCBI Official Full Name
adenosine A2b receptor
NCBI Official Synonym Full Names
adenosine A2b receptor
NCBI Official Symbol
ADORA2B
NCBI Official Synonym Symbols
ADORA2
UniProt Protein Name
Adenosine receptor A2b
Protein Family
Adenosine receptor
UniProt Gene Name
ADORA2B
UniProt Entry Name
AA2BR_HUMAN
NCBI Protein Information
adenosine receptor A2b

NCBI Summary for ADORA2b

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
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UniProt Comments for ADORA2b

ADORA2B: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: integral to plasma membrane; intracellular; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled

Biological Process: activation of MAPK activity; adenosine receptor signaling pathway; adenylate cyclase activation; cellular defense response; cellular protein metabolic process; cellular response to extracellular stimulus; excretion; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; JNK cascade; positive regulation of chemokine production; positive regulation of chronic inflammatory response to non-antigenic stimulus; positive regulation of guanylate cyclase activity; positive regulation of interleukin-6 production; positive regulation of mast cell degranulation; relaxation of vascular smooth muscle
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Research Articles on ADORA2b

1. The rs7208480 of ADORA2B as well as the haplotypes were not found to be associated with chronic heart failure susceptibility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADORA2b elisa kit

 Reference Product  PubMed Publications
 ADCY9 elisa kit  >1 publications with ADORA2b and ADCY9

Pathways associated with ADORA2b elisa kit

 Products by Pathway  Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram

Diseases associated with ADORA2b elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >21 publications with ADORA2b and Inflammation
 Cardiovascular Diseases Antibodies  >14 publications with ADORA2b and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >10 publications with ADORA2b and Disease Models, Animal
 Heart Diseases Antibodies  >8 publications with ADORA2b and Heart Diseases
 Hypertension Antibodies  >8 publications with ADORA2b and Hypertension
 Lung Injury Antibodies  >7 publications with ADORA2b and Lung Injury
 Nervous System Diseases Antibodies  >7 publications with ADORA2b and Nervous System Diseases
 Kidney Diseases Antibodies  >5 publications with ADORA2b and Kidney Diseases
 Edema Antibodies  >3 publications with ADORA2b and Edema
 Fibrosis Antibodies  >3 publications with ADORA2b and Fibrosis

Organs/Tissues associated with ADORA2b elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with ADORA2b and Blood
 Lung Antibodies  >11 publications with ADORA2b and Lung
 Heart Antibodies  >8 publications with ADORA2b and Heart
 Brain Antibodies  >6 publications with ADORA2b and Brain
 Intestine Antibodies  >3 publications with ADORA2b and Intestine
 Eye Antibodies  >3 publications with ADORA2b and Eye
 Stomach Antibodies  >2 publications with ADORA2b and Stomach
 Placenta Antibodies  >2 publications with ADORA2b and Placenta
 Ascites Antibodies  >1 publications with ADORA2b and Ascites
 Prostate Antibodies  >1 publications with ADORA2b and Prostate
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