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Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762625

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LPCAT3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LPCAT3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysophospholipid acyltransferase 5 (LPCAT3), ELISA Kit
Full Product Name
Human Lysophospholipid acyltransferase 5 ELISA Kit
Product Gene Name
LPCAT3 elisa kit
Product Synonym Names
LPCAT3 (Lysophospholipid acyltransferase 5)
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
46,765 Da
OMIM
611950
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of LPCAT3 elisa kit

Human LPCAT3 ELISA Kit – Cat# MBS762625

Typical Standard Curve of LPCAT3 elisa kit

Human LPCAT3 ELISA Kit, Typical Standard Curve – Cat# MBS762625

Other Notes

Small volumes of LPCAT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LPCAT3 purchase

MBS762625 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LPCAT3. The ELISA analytical biochemical technique of the MBS762625 kit is based on LPCAT3 antibody-LPCAT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LPCAT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LPCAT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LPCAT3. It may not necessarily be applicable to this product.

NCBI GI #
42542394
NCBI GeneID
10162
NCBI Accession #
NP_005759.4
UniProt Secondary Accession #
Q7KZS1; Q92980; Q9BW40; B2RDH0; B7Z3N3
UniProt Related Accession #
Q6P1A2
GenBank Nucleotide #
NM_005768.5
NCBI Official Full Name
lysophospholipid acyltransferase 5
NCBI Official Synonym Full Names
lysophosphatidylcholine acyltransferase 3
NCBI Official Symbol
LPCAT3
NCBI Official Synonym Symbols
C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
UniProt Protein Name
Lysophospholipid acyltransferase 5
UniProt Synonym Protein Names
1-acylglycerophosphocholine O-acyltransferase (EC:2.3.1.23); 1-acylglycerophosphoserine O-acyltransferase (EC:2.3.1.n6); Lysophosphatidylcholine acyltransferase; LPCAT; Lyso-PC acyltransferase; Lysophosphatidylcholine acyltransferase 3; Lyso-PC acyltransferase 3; Lysophosphatidylserine acyltransferase; LPSAT; Lyso-PS acyltransferase; Membrane-bound O-acyltransferase domain-containing protein 5; O-acyltransferase domain-containing protein 5
Protein Family
Lysophospholipid acyltransferase
UniProt Gene Name
LPCAT3
UniProt Synonym Gene Names
MBOAT5; OACT5; LPLAT 5; LPCAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; LPSAT; Lyso-PS acyltransferase; O-acyltransferase domain-containing protein 5
UniProt Entry Name
MBOA5_HUMAN
NCBI Protein Information
lysophospholipid acyltransferase 5

UniProt Comments for LPCAT3

MBOAT5: Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3- phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero- 3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn- glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Belongs to the membrane-bound acyltransferase family.

Protein type: EC 2.3.1.23; EC 2.3.1.n6; Membrane protein, multi-pass; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity

Biological Process: glycerophospholipid biosynthetic process; phospholipid metabolic process
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Research Articles on LPCAT3

1. LPCAT3 a key contributor to the human macrophages inflammatory response.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LPCAT3 elisa kit

 Reference Product  PubMed Publications
 LPCAT1 elisa kit  >3 publications with LPCAT3 and LPCAT1
 LPCAT2 elisa kit  >2 publications with LPCAT3 and LPCAT2

Pathways associated with LPCAT3 elisa kit

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 CDP-diacylglycerol Biosynthesis Pathway antibodies  CDP-diacylglycerol Biosynthesis Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Phospholipid Metabolism Pathway antibodies  Phospholipid Metabolism Pathway Diagram

Diseases associated with LPCAT3 elisa kit

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >10 publications with LPCAT3 and Fatty Liver
 Inflammation Antibodies  >5 publications with LPCAT3 and Inflammation
 Liver Diseases Antibodies  >2 publications with LPCAT3 and Liver Diseases
 Nervous System Diseases Antibodies  >1 publications with LPCAT3 and Nervous System Diseases
 Cholestasis Antibodies  >1 publications with LPCAT3 and Cholestasis
 Fibrosis Antibodies  >1 publications with LPCAT3 and Fibrosis
 Hypertrophy Antibodies  >1 publications with LPCAT3 and Hypertrophy
 Hepatomegaly Antibodies  >1 publications with LPCAT3 and Hepatomegaly
 Weight Loss Antibodies  >1 publications with LPCAT3 and Weight Loss

Organs/Tissues associated with LPCAT3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >14 publications with LPCAT3 and Liver
 Intestine Antibodies  >5 publications with LPCAT3 and Intestine
 Blood Antibodies  >4 publications with LPCAT3 and Blood
 Kidney Antibodies  >2 publications with LPCAT3 and Kidney
 Pancreas Antibodies  >1 publications with LPCAT3 and Pancreas
 Heart Antibodies  >1 publications with LPCAT3 and Heart
 Brain Antibodies  >1 publications with LPCAT3 and Brain
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