Neurogenic Locus Notch Homolog Protein 4 (NOTCH4) ELISA Kit for Human

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

NOTCH4: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; cytoplasmic vesicle; cytosol; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral to plasma membrane; nucleoplasm; nucleus; plasma membrane

Molecular Function: calcium ion binding; Notch binding; protein binding; protein heterodimerization activity; receptor activity

Biological Process: activation of Notch receptor target transcription factor; cell differentiation; cell fate determination; embryonic development; endothelial cell morphogenesis; gene expression; hemopoiesis; mammary gland development; morphogenesis of a branching structure; negative regulation of cell differentiation; negative regulation of endothelial cell differentiation; negative regulation of Notch signaling pathway; Notch receptor processing; Notch signaling pathway; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of transcription, DNA-dependent; regulation of protein localization; transcription initiation from RNA polymerase II promoter