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DNA Replication Factor Cdt1 (CDT1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763485

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CDT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CDT1 antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA replication factor Cdt1, ELISA Kit
Full Product Name
Human DNA replication factor Cdt1 ELISA Kit
Product Gene Name
CDT1 elisa kit
Product Synonym Names
CDT1/DNA replication factor Cdt1/Double parked homolog/DUP/RIS2
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of CDT1. No significant cross-reactivity or interference between CDT1 and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
60,390 Da
OMIM
605525
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of CDT1 elisa kit

Human CDT1 ELISA Kit – Cat# MBS763485

Other Notes

Small volumes of CDT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDT1 purchase

MBS763485 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA replication factor Cdt1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDT1. The ELISA analytical biochemical technique of the MBS763485 kit is based on CDT1 antibody-CDT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CDT1. It may not necessarily be applicable to this product.

NCBI GI #
188497689
NCBI GeneID
81620
NCBI Accession #
NP_112190.2
UniProt Secondary Accession #
Q86XX9; Q96CJ5; Q96GK5; Q96H67; Q96HE6; Q9BWM0
UniProt Related Accession #
Q9H211
GenBank Nucleotide #
NM_030928.3
NCBI Official Full Name
DNA replication factor Cdt1
NCBI Official Synonym Full Names
chromatin licensing and DNA replication factor 1
NCBI Official Symbol
CDT1
NCBI Official Synonym Symbols
DUP; RIS2
UniProt Protein Name
DNA replication factor Cdt1
UniProt Synonym Protein Names
Double parked homolog; DUP
Protein Family
Cell division cycle protein
UniProt Gene Name
CDT1
UniProt Synonym Gene Names
DUP
UniProt Entry Name
CDT1_HUMAN
NCBI Protein Information
DNA replication factor Cdt1

NCBI Summary for CDT1

The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]
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UniProt Comments for CDT1

CDT1: an essential licensing protein for DNA replication. Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Elevated in all human tumor cell lines analyzed. May function as an oncogene in mammals. Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; DNA-binding; DNA replication

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding

Biological Process: DNA replication; DNA replication checkpoint; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; mitotic cell cycle; regulation of DNA replication during S phase; regulation of DNA replication initiation

Disease: Meier-gorlin Syndrome 4
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Research Articles on CDT1

1. ATM silencing induced partial reduction in levels of Skp2, a component of SCF(Skp2) ubiquitin ligase that controls Cdt1 degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDT1 elisa kit

 Reference Product  PubMed Publications
 CDC6 elisa kit  >133 publications with CDT1 and CDC6
 GMNN elisa kit  >104 publications with CDT1 and GMNN
 PCNA elisa kit  >56 publications with CDT1 and PCNA
 MCM7 elisa kit  >30 publications with CDT1 and MCM7
 ORC1 elisa kit  >27 publications with CDT1 and ORC1
 CDK2 elisa kit  >26 publications with CDT1 and CDK2
 SKP2 elisa kit  >22 publications with CDT1 and SKP2
 MCM6 elisa kit  >21 publications with CDT1 and MCM6
 UBC elisa kit  >1 publications with CDT1 and UBC

Pathways associated with CDT1 elisa kit

 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 CDT1 Association With The CDC6:ORC:origin Complex Pathway antibodies  CDT1 Association With The CDC6:ORC:origin Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram

Diseases associated with CDT1 elisa kit

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >10 publications with CDT1 and Cell Transformation, Neoplastic
 Meier-Gorlin syndrome Antibodies  >10 publications with CDT1 and Meier-Gorlin syndrome
 Breast Neoplasms Antibodies  >7 publications with CDT1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >4 publications with CDT1 and Lung Neoplasms
 Precancerous Conditions Antibodies  >3 publications with CDT1 and Precancerous Conditions
 Neoplasms, Experimental Antibodies  >3 publications with CDT1 and Neoplasms, Experimental
 Hyperplasia Antibodies  >3 publications with CDT1 and Hyperplasia
 Carcinoma, Hepatocellular Antibodies  >2 publications with CDT1 and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >2 publications with CDT1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >2 publications with CDT1 and Cardiovascular Diseases

Organs/Tissues associated with CDT1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >11 publications with CDT1 and Bone
 Brain Antibodies  >11 publications with CDT1 and Brain
 Blood Antibodies  >10 publications with CDT1 and Blood
 Lung Antibodies  >9 publications with CDT1 and Lung
 Ovary Antibodies  >7 publications with CDT1 and Ovary
 Eye Antibodies  >6 publications with CDT1 and Eye
 Prostate Antibodies  >5 publications with CDT1 and Prostate
 Bone Marrow Antibodies  >5 publications with CDT1 and Bone Marrow
 Embryonic Tissue Antibodies  >4 publications with CDT1 and Embryonic Tissue
 Kidney Antibodies  >3 publications with CDT1 and Kidney
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