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Hyaluronidase-1 (HYAL1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763607

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HYAL1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HYAL1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hyaluronidase-1 (HYAL1), ELISA Kit
Full Product Name
Human Hyaluronidase-1 ELISA Kit
Product Gene Name
HYAL1 elisa kit
Product Synonym Names
HYAL1/Hyaluronidase-1/Hyal-1/Hyaluronoglucosaminidase-1/Lung carcinoma protein 1/LuCa-1
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
37,439 Da
OMIM
601492
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of HYAL1 elisa kit

Human HYAL1 ELISA Kit – Cat# MBS763607

Typical Standard Curve of HYAL1 elisa kit

Human HYAL1 ELISA Kit, Typical Standard Curve – Cat# MBS763607

Other Notes

Small volumes of HYAL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HYAL1 purchase

MBS763607 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hyaluronidase-1 (HYAL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HYAL1. The ELISA analytical biochemical technique of the MBS763607 kit is based on HYAL1 antibody-HYAL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HYAL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HYAL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HYAL1. It may not necessarily be applicable to this product.

NCBI GI #
24497566
NCBI GeneID
3373
NCBI Accession #
NP_695014.1
UniProt Secondary Accession #
Q6FH23; Q6PIZ6; Q7KYU2; Q7LE34; Q8NFK5; Q8NFK6; Q8NFK7; Q8NFK8; Q8NFK9; Q93013; Q9UKD5
UniProt Related Accession #
Q12794
GenBank Nucleotide #
NM_153282.2
NCBI Official Full Name
hyaluronidase-1 isoform 2
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 1
NCBI Official Symbol
HYAL1
NCBI Official Synonym Symbols
MPS9; NAT6; LUCA1; HYAL-1
UniProt Protein Name
Hyaluronidase-1
UniProt Synonym Protein Names
Hyaluronoglucosaminidase-1; Lung carcinoma protein 1; LuCa-1
Protein Family
Hyaluronidase
UniProt Gene Name
HYAL1
UniProt Synonym Gene Names
LUCA1; Hyal-1; LuCa-1
UniProt Entry Name
HYAL1_HUMAN
NCBI Protein Information
hyaluronidase-1

NCBI Summary for HYAL1

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for HYAL1

HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Hydrolase; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular space; lysosomal lumen; lysosome

Molecular Function: hyaluronan synthase activity; hyalurononglucosaminidase activity; transcription factor binding; viral receptor activity

Biological Process: carbohydrate metabolic process; cartilage development; chondroitin sulfate catabolic process; chondroitin sulfate metabolic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan catabolic process; hyaluronan metabolic process; inflammatory response; negative regulation of cell growth; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of growth; response to antibiotic; response to reactive oxygen species; response to virus

Disease: Mucopolysaccharidosis, Type Ix
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Research Articles on HYAL1

1. Study showed that reduced HYAL1 expression was associated with endometrial carcinoma aggressiveness, which further supported the role of hyaluronan degradation in cancer progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HYAL1 elisa kit

 Products by Pathway  Pathway Diagram
 CS/DS Degradation Pathway antibodies  CS/DS Degradation Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Hyaluronan Metabolism Pathway antibodies  Hyaluronan Metabolism Pathway Diagram

Diseases associated with HYAL1 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12 publications with HYAL1 and Inflammation
 Lung Neoplasms Antibodies  >12 publications with HYAL1 and Lung Neoplasms
 Hyaluronidase Deficiency Antibodies  >11 publications with HYAL1 and Hyaluronidase Deficiency
 Necrosis Antibodies  >8 publications with HYAL1 and Necrosis
 Neoplasms, Experimental Antibodies  >6 publications with HYAL1 and Neoplasms, Experimental
 Immune System Diseases Antibodies  >5 publications with HYAL1 and Immune System Diseases
 Urinary Bladder Neoplasms Antibodies  >5 publications with HYAL1 and Urinary Bladder Neoplasms
 Kidney Diseases Antibodies  >4 publications with HYAL1 and Kidney Diseases
 Liver Neoplasms Antibodies  >2 publications with HYAL1 and Liver Neoplasms
 Hyperplasia Antibodies  >2 publications with HYAL1 and Hyperplasia

Organs/Tissues associated with HYAL1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >23 publications with HYAL1 and Blood
 Lung Antibodies  >16 publications with HYAL1 and Lung
 Kidney Antibodies  >14 publications with HYAL1 and Kidney
 Prostate Antibodies  >13 publications with HYAL1 and Prostate
 Bladder Antibodies  >8 publications with HYAL1 and Bladder
 Vascular Antibodies  >7 publications with HYAL1 and Vascular
 Liver Antibodies  >7 publications with HYAL1 and Liver
 Muscle Antibodies  >6 publications with HYAL1 and Muscle
 Brain Antibodies  >4 publications with HYAL1 and Brain
 Connective Tissue Antibodies  >4 publications with HYAL1 and Connective Tissue
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