Anti-Cardiolipin Antibody IgM (ACA-IgM) ELISA Kit for Mouse

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Mouse ACA-IgM. Standards or samples are then added to the appropriate micro ELISA plate wells and combined to the specific antigen. Then a biotinylated detection antigen specific for Mouse ACA-IgM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACA-IgM, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ACA-IgM. You can calculate the concentration of Mouse ACA-IgM in the samples by comparing the OD of the samples to the standard curve.

Small volumes of ACA-IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763869 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the anti-Cardiolipin Antibody IgM (ACA-IgM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACA-IgM. The ELISA analytical biochemical technique of the MBS763869 kit is based on ACA-IgM antibody-ACA-IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACA-IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACA-IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).