Gastric Inhibitory Poly (GIP) ELISA Kit for Human

Name: Human GIP ELISA Kit; Gastric Inhibitory Poly; MyBioSource
Brand: MyBioSource
SKU: MBS764589
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764589

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GH antibody was used as detection antibodies. The standards, test samples… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Gastric inhibitory polypeptide (GIP), ELISA Kit

Full Product Name

Human Gastric inhibitory polypeptide ELISA Kit

Product Gene Name

GIP elisa kit

Product Synonym Names

GIP (gastric inhibitory polypeptide)/Glucose-dependent Insulinotropic Polypeptide/Incretin

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

46.875-3000pg/ml

Sensitivity

28.125pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of GH. No significant cross-reactivity or interference between GH and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

17,108 Da

OMIM

137240

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of GIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GIP purchase

MBS764589 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gastric inhibitory polypeptide (GIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GIP. The ELISA analytical biochemical technique of the MBS764589 kit is based on GIP antibody-GIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GIP. It may not necessarily be applicable to this product.

NCBI GI #

4758436

NCBI GeneID

2695

NCBI Accession #

NP_004114.1

UniProt Secondary Accession #

Q4VB42; Q6NTD3

UniProt Related Accession #

P09681

GenBank Nucleotide #

NM_004123.2

NCBI Official Full Name

gastric inhibitory polypeptide preproprotein

NCBI Official Synonym Full Names

gastric inhibitory polypeptide

NCBI Official Symbol

GIP

UniProt Protein Name

Gastric inhibitory polypeptide

UniProt Synonym Protein Names

Glucose-dependent insulinotropic polypeptide; Incretin hormone

Protein Family

Copia protein

UniProt Gene Name

GIP

UniProt Synonym Gene Names

GIP

UniProt Entry Name

GIP_HUMAN

NCBI Protein Information

gastric inhibitory polypeptide

NCBI Summary for GIP

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

UniProt Comments for GIP

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.3-q22

Cellular Component: cell soma; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: hormone activity; protein binding

Biological Process: adult locomotory behavior; cellular protein metabolic process; endocrine pancreas development; female pregnancy; memory; positive regulation of insulin secretion; regulation of insulin secretion; response to acidity; response to amino acid stimulus; response to axon injury; response to drug; response to glucose stimulus; response to lipid; response to organic cyclic substance; response to peptide hormone stimulus; response to selenium ion; response to starvation; sensory perception of pain; signal transduction

Research Articles on GIP

1. irisin and GIP might contribute to the development of polycystic ovary syndrome and may also represent novel polycystic ovary syndrome biomarkers

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GIP elisa kit

Reference Product	PubMed Publications
VIP elisa kit	>160 publications with GIP and VIP
GIPR elisa kit	>109 publications with GIP and GIPR
GLP1R elisa kit	>100 publications with GIP and GLP1R
IAPP elisa kit	>19 publications with GIP and IAPP
GPR119 elisa kit	>15 publications with GIP and GPR119
GCG elisa kit	>14 publications with GIP and GCG
GHRH elisa kit	>7 publications with GIP and GHRH
GCGR elisa kit	>6 publications with GIP and GCGR
SCT elisa kit	>5 publications with GIP and SCT
GATA4 elisa kit	>3 publications with GIP and GATA4

Pathways associated with GIP elisa kit

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Glucagon-type Ligand Receptors Pathway antibodies	Glucagon-type Ligand Receptors Pathway Diagram
Incretin Synthesis, Secretion, And Inactivation Pathway antibodies	Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Peptide Hormone Metabolism Pathway antibodies	Peptide Hormone Metabolism Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with GIP elisa kit

Disease Name	Pubmed Publications
Diabetes Mellitus, Type 2 Antibodies	>506 publications with GIP and Diabetes Mellitus, Type 2
Hyperglycemia Antibodies	>273 publications with GIP and Hyperglycemia
Weight Loss Antibodies	>123 publications with GIP and Weight Loss
Weight Gain Antibodies	>71 publications with GIP and Weight Gain
Inflammation Antibodies	>68 publications with GIP and Inflammation
Carcinoma Antibodies	>49 publications with GIP and Carcinoma
Neurologic Manifestations Antibodies	>47 publications with GIP and Neurologic Manifestations
Hyperplasia Antibodies	>45 publications with GIP and Hyperplasia
Kidney Diseases Antibodies	>33 publications with GIP and Kidney Diseases
Hypertension Antibodies	>32 publications with GIP and Hypertension

Organs/Tissues associated with GIP elisa kit

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>510 publications with GIP and Intestine
Stomach Antibodies	>161 publications with GIP and Stomach