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Disintegrin and Metalloproteinase Domain-Containing Protein 12 (ADAM12) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764756

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ADAM9/CORD9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Human ADAM9/CORD9 antibody was used as detection… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12), ELISA Kit
Full Product Name
Human Disintegrin and metalloproteinase domain-containing protein 12 ELISA Kit
Product Gene Name
ADAM12 elisa kit
Product Synonym Names
ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12)/Meltrin alpha/a disintegrin and metalloproteinase domain 12 (meltrin alpha)/ADAM 12/ADAM metallopeptidase domain 12/disintegrin and metalloproteinase domain-containing protein 12/MCMP/MCMPMltna/Meltrin-alpha/meltrin-alpha/MLTNA
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
80,263 Da
OMIM
602714
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of ADAM12 elisa kit

Human ADAM12 ELISA Kit – Cat# MBS764756

Typical Standard Curve of ADAM12 elisa kit

Human ADAM12 ELISA Kit, Typical Standard Curve – Cat# MBS764756

Other Notes

Small volumes of ADAM12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAM12 purchase

MBS764756 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM12. The ELISA analytical biochemical technique of the MBS764756 kit is based on ADAM12 antibody-ADAM12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADAM12. It may not necessarily be applicable to this product.

NCBI GI #
572882446
NCBI GeneID
8038
NCBI Accession #
NP_001275902.1
UniProt Secondary Accession #
O60470; Q5JRP0; Q5JRP1; Q6P9E3; Q6UWB0
UniProt Related Accession #
O43184
GenBank Nucleotide #
NM_001288973.1
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 12 isoform 3 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 12
NCBI Official Symbol
ADAM12
NCBI Official Synonym Symbols
MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 12
UniProt Synonym Protein Names
Meltrin-alpha
Protein Family
Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name
ADAM12
UniProt Synonym Gene Names
MLTN; ADAM 12
UniProt Entry Name
ADA12_HUMAN
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 12

NCBI Summary for ADAM12

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
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UniProt Comments for ADAM12

ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: EC 3.4.24.-; Protease; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: extracellular region; integral to membrane; mitochondrion; nucleoplasm; plasma membrane

Molecular Function: metalloendopeptidase activity; metallopeptidase activity; protein binding; SH3 domain binding; zinc ion binding

Biological Process: cell adhesion; epidermal growth factor receptor signaling pathway; myoblast fusion; proteolysis
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Research Articles on ADAM12

1. The ADAM12-L 3'UTR is a direct target of miR-29 and miR-200 family members.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADAM12 elisa kit

 Reference Product  PubMed Publications
 ADAM17 elisa kit  >30 publications with ADAM12 and ADAM17
 EGF elisa kit  >28 publications with ADAM12 and EGF
 ADAM10 elisa kit  >26 publications with ADAM12 and ADAM10
 EGFR elisa kit  >13 publications with ADAM12 and EGFR
 SRC elisa kit  >10 publications with ADAM12 and SRC
 DLL1 elisa kit  >3 publications with ADAM12 and DLL1
 IGFBP3 elisa kit  >2 publications with ADAM12 and IGFBP3
 SH3D19 elisa kit  >1 publications with ADAM12 and SH3D19
 PACSIN3 elisa kit  >1 publications with ADAM12 and PACSIN3

Pathways associated with ADAM12 elisa kit

 Products by Pathway  Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By EGFR Pathway antibodies  Signaling By EGFR Pathway Diagram
 Syndecan-4-mediated Signaling Events Pathway antibodies  Syndecan-4-mediated Signaling Events Pathway Diagram

Diseases associated with ADAM12 elisa kit

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >25 publications with ADAM12 and Necrosis
 Breast Neoplasms Antibodies  >24 publications with ADAM12 and Breast Neoplasms
 Fibrosis Antibodies  >12 publications with ADAM12 and Fibrosis
 Inflammation Antibodies  >12 publications with ADAM12 and Inflammation
 Disease Models, Animal Antibodies  >10 publications with ADAM12 and Disease Models, Animal
 Lung Neoplasms Antibodies  >9 publications with ADAM12 and Lung Neoplasms
 Hypertrophy Antibodies  >7 publications with ADAM12 and Hypertrophy
 Cardiomegaly Antibodies  >7 publications with ADAM12 and Cardiomegaly
 Growth Disorders Antibodies  >7 publications with ADAM12 and Growth Disorders
 Neoplasms, Experimental Antibodies  >5 publications with ADAM12 and Neoplasms, Experimental

Organs/Tissues associated with ADAM12 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >57 publications with ADAM12 and Muscle
 Vascular Antibodies  >31 publications with ADAM12 and Vascular
 Placenta Antibodies  >31 publications with ADAM12 and Placenta
 Embryonic Tissue Antibodies  >25 publications with ADAM12 and Embryonic Tissue
 Bone Antibodies  >24 publications with ADAM12 and Bone
 Brain Antibodies  >18 publications with ADAM12 and Brain
 Lung Antibodies  >15 publications with ADAM12 and Lung
 Heart Antibodies  >13 publications with ADAM12 and Heart
 Liver Antibodies  >12 publications with ADAM12 and Liver
 Connective Tissue Antibodies  >12 publications with ADAM12 and Connective Tissue
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