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Tyrosine-Protein Phosphatase Non-Receptor Type 1 (PTPN1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764808

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ptpn1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ptpn1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tyrosine-protein phosphatase non-receptor type 1 (Ptpn1), ELISA Kit
Full Product Name
Mouse Tyrosine-protein phosphatase non-receptor type 1 ELISA Kit
Product Gene Name
Ptpn1 elisa kit
Product Synonym Names
Ptpn1/Tyrosine-protein phosphatase non-receptor type 1/Protein-tyrosine phosphatase 1B/PTP-1B/PTP1B/protein tyrosine phosphatase 1B/protein tyrosine phosphatase; non-receptor type 1/PTP1Bprotein tyrosine phosphatase; placental
Species Reactivity
Mouse
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
49,593 Da
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Ptpn1 elisa kit

Mouse PTPN1 ELISA Kit – Cat# MBS764808

Typical Standard Curve of Ptpn1 elisa kit

Mouse PTPN1 ELISA Kit, Typical Standard Curve – Cat# MBS764808

Other Notes

Small volumes of Ptpn1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ptpn1 purchase

MBS764808 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosine-protein phosphatase non-receptor type 1 (Ptpn1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ptpn1. The ELISA analytical biochemical technique of the MBS764808 kit is based on Ptpn1 antibody-Ptpn1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ptpn1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ptpn1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Ptpn1. It may not necessarily be applicable to this product.

NCBI GI #
133505845
NCBI GeneID
19246
NCBI Accession #
NP_035331.3
UniProt Secondary Accession #
Q60840; Q62131; Q64498; Q99JS1
UniProt Related Accession #
P35821
GenBank Nucleotide #
NM_011201.3
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 1
NCBI Official Symbol
Ptpn1
NCBI Official Synonym Symbols
PTP1B; PTP-1B; PTP-HA2
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 1
UniProt Synonym Protein Names
Protein-tyrosine phosphatase 1B; PTP-1B; Protein-tyrosine phosphatase HA2; PTP-HA2
Protein Family
Tyrosine-protein phosphatase non-receptor
UniProt Gene Name
Ptpn1
UniProt Synonym Gene Names
PTP-1B; PTP-HA2
UniProt Entry Name
PTN1_MOUSE
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 1

UniProt Comments for Ptpn1

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Protein phosphatase, tyrosine (non-receptor); Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.48

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; early endosome; endoplasmic reticulum; membrane; plasma membrane

Molecular Function: enzyme binding; ephrin receptor binding; hydrolase activity; insulin receptor binding; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein kinase binding; protein phosphatase 2A binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; activation of JNK activity; dephosphorylation; insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid dephosphorylation; regulation of endocytosis; regulation of insulin receptor signaling pathway; regulation of intracellular protein transport; regulation of signal transduction; unfolded protein response
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Research Articles on Ptpn1

1. This study presents PTP1B as a mechanism-based therapeutic target for RTT, validating a unique strategy for treating the disease by modifying signal transduction pathways with small-molecule drugs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Ptpn1 elisa kit

 Reference Product  PubMed Publications
 Src elisa kit  >78 publications with Ptpn1 and Src
 Irs1 elisa kit  >32 publications with Ptpn1 and Irs1
 Jak2 elisa kit  >28 publications with Ptpn1 and Jak2
 Egfr elisa kit  >22 publications with Ptpn1 and Egfr
 Akt1 elisa kit  >9 publications with Ptpn1 and Akt1
 Tyk2 elisa kit  >7 publications with Ptpn1 and Tyk2
 Insr elisa kit  >5 publications with Ptpn1 and Insr
 Jak1 elisa kit  >4 publications with Ptpn1 and Jak1
 Bcar1 elisa kit  >4 publications with Ptpn1 and Bcar1
 Grb2 elisa kit  >2 publications with Ptpn1 and Grb2

Pathways associated with Ptpn1 elisa kit

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Growth Hormone Receptor Signaling Pathway antibodies  Growth Hormone Receptor Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Resistance Pathway antibodies  Insulin Resistance Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with Ptpn1 elisa kit

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >170 publications with Ptpn1 and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >84 publications with Ptpn1 and Diabetes Mellitus, Type 2
 Disease Models, Animal Antibodies  >54 publications with Ptpn1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >45 publications with Ptpn1 and Cardiovascular Diseases
 Hyperglycemia Antibodies  >37 publications with Ptpn1 and Hyperglycemia
 Inflammation Antibodies  >30 publications with Ptpn1 and Inflammation
 Diabetes Mellitus, Experimental Antibodies  >26 publications with Ptpn1 and Diabetes Mellitus, Experimental
 Necrosis Antibodies  >25 publications with Ptpn1 and Necrosis
 Weight Gain Antibodies  >23 publications with Ptpn1 and Weight Gain
 Breast Neoplasms Antibodies  >21 publications with Ptpn1 and Breast Neoplasms

Organs/Tissues associated with Ptpn1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >138 publications with Ptpn1 and Blood
 Muscle Antibodies  >99 publications with Ptpn1 and Muscle
 Liver Antibodies  >95 publications with Ptpn1 and Liver
 Brain Antibodies  >50 publications with Ptpn1 and Brain
 Connective Tissue Antibodies  >33 publications with Ptpn1 and Connective Tissue
 Heart Antibodies  >31 publications with Ptpn1 and Heart
 Kidney Antibodies  >21 publications with Ptpn1 and Kidney
 Lung Antibodies  >17 publications with Ptpn1 and Lung
 Pancreas Antibodies  >16 publications with Ptpn1 and Pancreas
 Bone Antibodies  >12 publications with Ptpn1 and Bone
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