Cytochrome P450 2c19 (CYP2C19) ELISA Kit for Human

Background: Cytochrome P450 2C19 (CYP2C19) is a drug-metabolizing enzyme involved in the metabolism of several clinically important drugs including the platelet aggregation inhibitor clopidogrel, the tricyclic antidepressants amitriptyline and nortriptyline, the selective serotonin reuptake inhibitors citalopram and sertraline, and some proton pump inhibitors including omeprazole and pantoprazole. CYP2C19 genotype information can be utilized to predict CYP2C19 metabolic phenotype, which can be used as an aid in determining a therapeutic strategy for drugs that are metabolized by CYP2C19.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CYP2C19 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti CYP2C19 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CYP2C19 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CYP2C19 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of CYP2C19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS765749 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 2C19 (CYP2C19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP2C19. The ELISA analytical biochemical technique of the MBS765749 kit is based on CYP2C19 antibody-CYP2C19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2C19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2C19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).