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Aurora B Kinase (AURKB) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555614

MBS555614 is an antibody targeting AURKB. Also known as Aurora B kinase, Monoclonal Antibody, Mouse. Reactive in Human, Cow, Pig, Chicken, Rat, Mouse. Host: Mouse. More details

$520 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Aurora B kinase (AURKB), Antibody
Full Product Name
Aurora B kinase, Monoclonal Antibody, Mouse
Product Gene Name
anti-AURKB antibody
Isotype
IgG
Host
Mouse
Species Reactivity
Human, Cow, Pig, Chicken, Rat, Mouse
Form/Format
100 ul liquid 10 mM Sodium Azide.
Purity/Purification
Protein G purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2°C-8°C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
OMIM
604970
Immunogen
Full length recombinant human Aurora B protein expressed in and purified from E. coli

Product Categories/Family for anti-AURKB antibody

Aurora B kinase
Applications Tested/Suitable for anti-AURKB antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-AURKB antibody
Immunofluorescence: 1:1000
Immunochemisity: 1:1000
Immunohistochemistry: 1:1000
Western Blot: 1:1000

Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Testing Data of anti-AURKB antibody

Image: HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red). Blue is a DNA stain.
Mouse Anti-AURKB Antibody – Cat# MBS555614

Testing Data of anti-AURKB antibody

Image: Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with MBS555614. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.
Mouse Anti-AURKB Antibody, Testing Data – Cat# MBS555614

Other Notes

Small volumes of anti-AURKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AURKB. It may not necessarily be applicable to this product.

NCBI GI #
927644272
NCBI GeneID
9212
NCBI Accession #
NP_001300884.1
UniProt Secondary Accession #
O14630; O60446; O95083; Q96DV5; Q9UQ46; B4DNM4; C7G533; C7G534; C7G535; D3DTR4; J9JID1
UniProt Related Accession #
Q96GD4
GenBank Nucleotide #
NM_001313955.1
NCBI Official Full Name
aurora kinase B isoform 7
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
UniProt Protein Name
Aurora kinase B
UniProt Synonym Protein Names
Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B
Protein Family
Aurora kinase
UniProt Gene Name
AURKB
UniProt Synonym Gene Names
AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2; Aurora-related kinase 2
UniProt Entry Name
AURKB_HUMAN
NCBI Protein Information
aurora kinase B

UniProt Comments for AURKB

AurB: a member of the AUR family of kinases. May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Expressed during S and G2/M phase and expression is upregulated in cancer cells during M phase. Localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis. Colocalizes with gamma tubulin in the mid-body. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Overexpressed in colorectal and other cancer cell lines and thought to cause aneuploidy via histone phosphorylation.

Protein type: AUR family; EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: condensed nuclear chromosome, pericentric region; cytosol; intercellular bridge; kinetochore; midbody; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole centrosome

Molecular Function: histone serine kinase activity; protein binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: abscission; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; attachment of spindle microtubules to kinetochore; histone modification; negative regulation of B cell apoptosis; negative regulation of cytokinesis; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cytokinesis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein sumoylation; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of chromosome segregation; sister chromatid cohesion; spindle midzone assembly involved in mitosis; spindle organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AURKB antibody

 Reference Product  PubMed Publications
 INCENP antibody  >101 publications with AURKB and INCENP
 BIRC5 antibody  >87 publications with AURKB and BIRC5
 CDCA8 antibody  >29 publications with AURKB and CDCA8
 NDC80 antibody  >25 publications with AURKB and NDC80
 CENPA antibody  >5 publications with AURKB and CENPA
 KLHL9 antibody  >3 publications with AURKB and KLHL9
 KLHL13 antibody  >3 publications with AURKB and KLHL13
 MAPRE1 antibody  >3 publications with AURKB and MAPRE1
 RACGAP1 antibody  >1 publications with AURKB and RACGAP1

Pathways associated with anti-AURKB antibody

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Aurora C Signaling Pathway antibodies  Aurora C Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram

Diseases associated with anti-AURKB antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >36 publications with AURKB and Breast Neoplasms
 Adenocarcinoma Antibodies  >33 publications with AURKB and Adenocarcinoma
 Nervous System Diseases Antibodies  >23 publications with AURKB and Nervous System Diseases
 Lung Neoplasms Antibodies  >23 publications with AURKB and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >21 publications with AURKB and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >20 publications with AURKB and Neoplasm Metastasis
 Pancreatic Neoplasms Antibodies  >15 publications with AURKB and Pancreatic Neoplasms
 Ovarian Neoplasms Antibodies  >15 publications with AURKB and Ovarian Neoplasms
 Carcinoma, Hepatocellular Antibodies  >15 publications with AURKB and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >15 publications with AURKB and Disease Models, Animal

Organs/Tissues associated with anti-AURKB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >39 publications with AURKB and Blood
 Lung Antibodies  >34 publications with AURKB and Lung
 Brain Antibodies  >26 publications with AURKB and Brain
 Liver Antibodies  >25 publications with AURKB and Liver
 Bone Antibodies  >21 publications with AURKB and Bone
 Prostate Antibodies  >18 publications with AURKB and Prostate
 Bone Marrow Antibodies  >15 publications with AURKB and Bone Marrow
 Lymph Antibodies  >13 publications with AURKB and Lymph
 Lymph Node Antibodies  >13 publications with AURKB and Lymph Node
 Testis Antibodies  >12 publications with AURKB and Testis
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