PSD95 (DLG4) Antibody – Mouse Monoclonal

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Protein type: Adaptor/scaffold

Cellular Component: dendrite cytoplasm; cortical cytoskeleton; voltage-gated potassium channel complex; endoplasmic reticulum; basolateral plasma membrane; postsynaptic density; dendrite; dendritic spine; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; glutamate receptor binding; beta-1 adrenergic receptor binding; acetylcholine receptor binding; protein phosphatase binding; protein kinase binding; PDZ domain binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; protein complex binding; D1 dopamine receptor binding; receptor binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; regulation of long-term neuronal synaptic plasticity; elevation of cytosolic calcium ion concentration; establishment of protein localization; synaptic vesicle maturation; protein complex assembly; social behavior; neuronal ion channel clustering; neuromuscular process controlling balance; positive regulation of synaptic transmission; nucleotide phosphorylation; negative regulation of receptor internalization