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Sodium-Iodide Symporter (SLC5A5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS802004

Background Info: Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160kDa, and degradation products at ~30 kDa, and ~15kDa.Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively… More details

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Product Overview

Online Datasheet in PDF
Product Name
Sodium-Iodide Symporter (SLC5A5), Monoclonal Antibody
Full Product Name
Sodium-Iodide Symporter Antibody: ATTO 594
Product Gene Name
anti-SLC5A5 antibody
Product Synonym Names
NIS; SLC5A5; solute carrier family 5; Na (+)I(-) cotransporter
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14F
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: Sodium-Iodide Symporter Clone #14F (MBS801448)
ATTO 594 Conjugated Antibody: Sodium-Iodide Symporter Clone #14F (MBS802004)
Matching Pairs
Unconjugated Antibody: N/A (MBS802004)
ATTO 594 Conjugated Antibody: Sodium-Iodide Symporter Clone #14F (MBS802004)
Molecular Weight
68,666 Da
OMIM
601843
3D Structure
ModBase 3D Structure for Q92911
Conjugate
ATTO 594
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-SLC5A5 antibody

Transporters
Applications Tested/Suitable for anti-SLC5A5 antibody
Western Blot (WB)
Application Notes for anti-SLC5A5 antibody
1:1000 (WB)

Immunohistochemistry (IHC) of anti-SLC5A5 antibody

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Mouse Anti-SLC5A5 Monoclonal Antibody – Cat# MBS802004

Western Blot (WB) of anti-SLC5A5 antibody

Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000.
Mouse Anti-SLC5A5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS802004

Immunohistochemistry (IHC) of anti-SLC5A5 antibody

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Mouse Anti-SLC5A5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS802004

Immunohistochemistry (IHC) of anti-SLC5A5 antibody

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Mouse Anti-SLC5A5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS802004

Immunohistochemistry (IHC) of anti-SLC5A5 antibody

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Mouse Anti-SLC5A5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS802004

Product References and Citations for anti-SLC5A5 antibody

1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.

Other Notes

Small volumes of anti-SLC5A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC5A5 purchase

MBS802004 is an antibody targeting SLC5A5. Also known as Sodium-Iodide Symporter Antibody: ATTO 594. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC5A5. It may not necessarily be applicable to this product.

NCBI GI #
4507035
NCBI GeneID
6528
NCBI Accession #
NP_000444.1
UniProt Primary Accession #
Q92911
UniProt Secondary Accession #
O43702; Q2M335; Q9NYB6
UniProt Related Accession #
Q92911
GenBank Nucleotide #
NM_000453.2
NCBI Official Full Name
sodium/iodide cotransporter
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/iodide cotransporter), member 5
NCBI Official Symbol
SLC5A5
NCBI Official Synonym Symbols
NIS; TDH1
UniProt Protein Name
Sodium/iodide cotransporter
UniProt Synonym Protein Names
Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5
Protein Family
Sodium/iodide cotransporter
UniProt Gene Name
SLC5A5
UniProt Synonym Gene Names
NIS; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter
UniProt Entry Name
SC5A5_HUMAN
NCBI Protein Information
sodium/iodide cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; solute carrier family 5 (sodium iodide symporter), member 5

NCBI Summary for SLC5A5

This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]
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UniProt Comments for SLC5A5

SLC5A5: Mediates iodide uptake in the thyroid gland. Defects in SLC5A5 are the cause of thyroid dyshormonogenesis 1 (TDH1). A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: integral to membrane; plasma membrane; nucleus; vesicle

Molecular Function: iodide transmembrane transporter activity; sodium:iodide symporter activity

Biological Process: iodide transport; transport; ion transport; thyroid hormone generation; transmembrane transport

Disease: Thyroid Dyshormonogenesis 1
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Research Articles on SLC5A5

1. this study identified a new distal enhancer, NIS distal enhancer, that regulates gene expression through DNA methylation in thyroid cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC5A5 antibody

 Reference Product  PubMed Publications
 TPO antibody  >5 publications with SLC5A5 and TPO
 DUOX2 antibody  >4 publications with SLC5A5 and DUOX2
 TSHR antibody  >4 publications with SLC5A5 and TSHR
 DUOXA2 antibody  >3 publications with SLC5A5 and DUOXA2
 SLC26A4 antibody  >2 publications with SLC5A5 and SLC26A4
 IYD antibody  >2 publications with SLC5A5 and IYD
 SLC5A8 antibody  >1 publications with SLC5A5 and SLC5A8

Pathways associated with anti-SLC5A5 antibody

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Organic Anion Transporters Pathway antibodies  Organic Anion Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroxine (Thyroid Hormone) Production Pathway antibodies  Thyroxine (Thyroid Hormone) Production Pathway Diagram
 Thyroxine Biosynthesis Pathway antibodies  Thyroxine Biosynthesis Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-SLC5A5 antibody

 Disease Name  Pubmed Publications
 Thyroid Neoplasms Antibodies  >6 publications with SLC5A5 and Thyroid Neoplasms
 Thyroid Diseases Antibodies  >5 publications with SLC5A5 and Thyroid Diseases
 Breast Neoplasms Antibodies  >2 publications with SLC5A5 and Breast Neoplasms
 Prenatal Injuries Antibodies  >1 publications with SLC5A5 and Prenatal Injuries
 Liver Diseases Antibodies  >1 publications with SLC5A5 and Liver Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with SLC5A5 and Prenatal Exposure Delayed Effects
 Prostatic Neoplasms Antibodies  >1 publications with SLC5A5 and Prostatic Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with SLC5A5 and Carcinoma, Hepatocellular
 Thyroid Dyshormonogenesis 1 Antibodies  >1 publications with SLC5A5 and Thyroid Dyshormonogenesis 1
 Cholangiocarcinoma Antibodies  >1 publications with SLC5A5 and Cholangiocarcinoma

Organs/Tissues associated with anti-SLC5A5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SLC5A5 and Brain
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