Mouse Anti-AMIGO1 Monoclonal Antibody – Cat# MBS802414

Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.

AMIGO-1 (AMIGO1) Antibody – Mouse Monoclonal

Name: AMIGO1 Antibody; Monoclonal; Mouse; Mouse/Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS802414
Price: 570 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS802414

Background Info: Detects 60-80kDa depending on maturity/glycosylation. Does not bind AMIGO-2 or 3.Scientific Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

AMIGO-1 (AMIGO1), Monoclonal Antibody

Full Product Name

AMIGO-1 Antibody: RPE

Product Gene Name

anti-AMIGO1 antibody

Product Synonym Names

AMIGO 1; AMIGO1; Adhesion molecule with Ig like domain 1; Amphoterin-induced protein 1; Alivin-2; Alivin 2; Ali2; AMIGO; KIAA1163; Amphoterin induced gene and ORF (Amigo); Amphoterin induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6

Clonality

Monoclonal

Isotype

IgG1

Clone Number

S86-36

Host

Mouse

Species Reactivity

Mouse, Human, Rat

Form/Format

Protein G Purified

Concentration

1mg/mL (lot specific)

Preparation and Storage

-20 degree C

Matching Pairs

Unconjugated Antibody: AMIGO-1 Clone #S86-36 (MBS801032)
RPE Conjugated Antibody: AMIGO-1 Clone #S86-36 (MBS802414)

Matching Pairs

Unconjugated Antibody: N/A (MBS802414)
RPE Conjugated Antibody: AMIGO-1 Clone #S86-36 (MBS802414)

Molecular Weight

55,239 Da

OMIM

615689

3D Structure

ModBase 3D Structure for Q86WK6

Conjugate

RPE

Storage Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-AMIGO1 antibody

Neuroscience

Applications Tested/Suitable for anti-AMIGO1 antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Application Notes for anti-AMIGO1 antibody

1:1000 (WB)

Product References and Citations for anti-AMIGO1 antibody

1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.

Other Notes

Small volumes of anti-AMIGO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMIGO1 purchase

MBS802414 is an antibody targeting AMIGO1. Also known as AMIGO-1 Antibody: RPE. Reactive in Mouse, Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AMIGO1. It may not necessarily be applicable to this product.

NCBI GI #

153791466

NCBI GeneID

57463

NCBI Accession #

NP_065754.2

UniProt Primary Accession #

Q86WK6

UniProt Secondary Accession #

Q8IW71; Q9ULQ7; B4DIM3

UniProt Related Accession #

Q86WK6

GenBank Nucleotide #

NM_020703.2

NCBI Official Full Name

amphoterin-induced protein 1

NCBI Official Synonym Full Names

adhesion molecule with Ig-like domain 1

NCBI Official Symbol

AMIGO1

NCBI Official Synonym Symbols

ALI2; AMIGO; AMIGO-1

UniProt Protein Name

Amphoterin-induced protein 1

UniProt Synonym Protein Names

AMIGO-1; Alivin-2

Protein Family

Amphoterin-induced protein

UniProt Gene Name

AMIGO1

UniProt Synonym Gene Names

ALI2; AMIGO; KIAA1163

UniProt Entry Name

AMGO1_HUMAN

NCBI Protein Information

amphoterin-induced protein 1; alivin-2; amphoterin-induced gene and ORF

UniProt Comments for AMIGO1

AMIGO1: Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Belongs to the immunoglobulin superfamily. AMIGO family.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: axon; plasma membrane; integral to membrane

Biological Process: heterophilic cell adhesion; myelination; axonogenesis; axonal fasciculation; positive regulation of axonogenesis; brain development; homophilic cell adhesion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AMIGO1 antibody

Reference Product	PubMed Publications
HMGB1 antibody	>3 publications with AMIGO1 and HMGB1
AMIGO2 antibody	>1 publications with AMIGO1 and AMIGO2
AMIGO3 antibody	>1 publications with AMIGO1 and AMIGO3

Organs/Tissues associated with anti-AMIGO1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with AMIGO1 and Brain