Rhodopsin (RHO) Antibody – Mouse Monoclonal

Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Binds specifically to the N-terminus of Rhodopsin.

Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
1. Cheng, C.L., Djajadi, H. and Molday, R.S. (2012). Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy. Methods in Molecular Biology. 935, 185-199. doi: 10.1007/978-1-62703-080-9_12

Small volumes of anti-RHO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS803129 is an antibody targeting RHO. Also known as Rhodopsin Antibody: Biotin. Reactive in All mammalian species (Human, Mice, Rat, Bovine, Pig etc.), most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.. Host: Mouse.