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KCNC4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS803140

Background Info: Detects ~70kDa (100kDa in brain due to glycosylation)Scientific Background: Kv3.4, a member of the Shaw subfamily, may play important roles in maintaining normal function of the corneal epithelium (3). Kv3.4 is also over-expressed in the… More details

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Product Overview

Product Name
Kv3.4 (Kcnc4), Monoclonal Antibody
Full Product Name
Kv3.4 Antibody
Product Gene Name
anti-Kcnc4 antibody
Product Synonym Names
Raw3; Voltage-gated potassium channel subunit Kv3.4; Potassium voltage-gated channel subfamily C member 4
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S72-16
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
68,435 Da
3D Structure
ModBase 3D Structure for Q63734
Certificate of Analysis
1ug/mL was sufficient for detection of Kv3.4 in 10ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Kcnc4 antibody

Ion Channels; Neuroscience
Applications Tested/Suitable for anti-Kcnc4 antibody
Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-Kcnc4 antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Kcnc4 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli .
Mouse Anti-KCNC4 Monoclonal Antibody – Cat# MBS803140

Western Blot (WB) of anti-Kcnc4 antibody

Western Blot analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-KCNC4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS803140

Immunohistochemistry (IHC) of anti-Kcnc4 antibody

Immunohistochemistry analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrin-like staining.
Mouse Anti-KCNC4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS803140

Product References and Citations for anti-Kcnc4 antibody

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Wang L., Fyffe R.E.W., and Lu L. (2004) Investigative Ophthalmology and Visual Science. 45: 1796-1803. 4. Angulo E., et al. (2004) J Neurochem. 91(3): 547-557.

Other Notes

Small volumes of anti-Kcnc4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Kcnc4 purchase

MBS803140 is an antibody targeting Kcnc4. Also known as Kv3.4 Antibody. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Kcnc4. It may not necessarily be applicable to this product.

NCBI GI #
221218994
NCBI GeneID
684516
NCBI Accession #
NP_001116248.1
UniProt Primary Accession #
Q63734
UniProt Related Accession #
Q63734
GenBank Nucleotide #
NM_001122776.1
NCBI Official Full Name
potassium voltage-gated channel subfamily C member 4
NCBI Official Synonym Full Names
potassium voltage gated channel, Shaw-related subfamily, member 4
NCBI Official Symbol
Kcnc4
UniProt Protein Name
Potassium voltage-gated channel subfamily C member 4
UniProt Synonym Protein Names
Raw3; Voltage-gated potassium channel subunit Kv3.4
Protein Family
Potassium voltage-gated channel subfamily
UniProt Gene Name
Kcnc4
UniProt Entry Name
KCNC4_RAT
NCBI Protein Information
potassium voltage-gated channel subfamily C member 4; raw3; voltage-gated potassium channel subunit Kv3.4; potassium voltage-gated channel subfamily C member 4 (Voltage-gated potassium channel subunit Kv3.4) (Raw3)

UniProt Comments for Kcnc4

Kv3.4: This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral

Cellular Component: axon; cell soma; dendrite; integral to membrane; nerve terminal; neuromuscular junction; plasma membrane; synaptic vesicle; voltage-gated potassium channel complex

Molecular Function: delayed rectifier potassium channel activity; molecular_function; voltage-gated potassium channel activity

Biological Process: forebrain development; protein homooligomerization; regulation of neurotransmitter secretion
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Research Articles on Kcnc4

1. the 15-lipoxygenase pathway via its metabolite, 15-hydroxyeicosatetraenoic acid , seems to play a role in the down-regulation of Kv3.4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Kcnc4 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >4 publications with Kcnc4 and Disease Models, Animal
 Epilepsy Antibodies  >2 publications with Kcnc4 and Epilepsy
 Mental Disorders Antibodies  >2 publications with Kcnc4 and Mental Disorders
 Dementia Antibodies  >2 publications with Kcnc4 and Dementia
 Carcinoma Antibodies  >2 publications with Kcnc4 and Carcinoma
 Seizures Antibodies  >1 publications with Kcnc4 and Seizures
 Acidosis Antibodies  >1 publications with Kcnc4 and Acidosis
 Arrhythmias, Cardiac Antibodies  >1 publications with Kcnc4 and Arrhythmias, Cardiac
 Alcoholism Antibodies  >1 publications with Kcnc4 and Alcoholism
 Epilepsy, Temporal Lobe Antibodies  >1 publications with Kcnc4 and Epilepsy, Temporal Lobe

Organs/Tissues associated with anti-Kcnc4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with Kcnc4 and Brain
 Nerve Antibodies  >1 publications with Kcnc4 and Nerve
 Eye Antibodies  >1 publications with Kcnc4 and Eye
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