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Thorase/Atad1 (ATAD1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS803878

Background Info: Detects ~40kDa.Scientific Background: ATPase family, AAA domain containing 1. Has a length of 361 amino acids (1). More details

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Product Overview

Product Name
Thorase/Atad1, Monoclonal Antibody
Full Product Name
Thorase/Atad1 Antibody: ATTO 390
Product Gene Name
anti-Atad1 antibody
Product Synonym Names
ATPase family AAA domain containing 1; FLJ14600; FNP001; AFDC1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S125-10
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
40,744 Da
3D Structure
ModBase 3D Structure for Q9D5TO
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Atad1 antibody

Ion Channels; Neuroscience
Applications Tested/Suitable for anti-Atad1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes for anti-Atad1 antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Atad1 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and accumulates around nucleus.
Mouse Anti-ATAD1 Monoclonal Antibody – Cat# MBS803878

Western Blot (WB) of anti-Atad1 antibody

Western Blot analysis of Rat brain membrane lysate showing detection of ATAD1 ATPase protein using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-ATAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS803878

Immunohistochemistry (IHC) of anti-Atad1 antibody

Immunohistochemistry analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining in all basal cells.
Mouse Anti-ATAD1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS803878

Product References and Citations for anti-Atad1 antibody

1. Genes to Systems Breast Cancer Database (G2SBC): www.itb.cnr.it/breastcancer/php/geneReport.php?id=84896

Other Notes

Small volumes of anti-Atad1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Atad1 purchase

MBS803878 is an antibody targeting Atad1. Also known as Thorase/Atad1 Antibody: ATTO 390. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Atad1. It may not necessarily be applicable to this product.

NCBI GI #
31560168
NCBI GeneID
67979
NCBI Accession #
NP_080763.2
UniProt Primary Accession #
Q9D5TO
UniProt Secondary Accession #
Q3U8V2; Q9D7A4
UniProt Related Accession #
Q9D5T0
GenBank Nucleotide #
NM_026487.3
NCBI Official Full Name
ATPase family AAA domain-containing protein 1
NCBI Official Synonym Full Names
ATPase family, AAA domain containing 1
NCBI Official Symbol
Atad1
NCBI Official Synonym Symbols
Thorase; AW107648; 4921525H23Rik
UniProt Protein Name
ATPase family AAA domain-containing protein 1
UniProt Synonym Protein Names
Thorase
Protein Family
ATPase family AAA domain-containing protein
UniProt Gene Name
Atad1
UniProt Entry Name
ATAD1_MOUSE
NCBI Protein Information
ATPase family AAA domain-containing protein 1

UniProt Comments for Atad1

ATAD1: ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA- stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent. Belongs to the AAA ATPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; EC 3.6.1.3

Cellular Component: peroxisomal membrane; postsynaptic membrane; mitochondrion; membrane; plasma membrane; synapse; peroxisome; cell junction; nucleus

Molecular Function: protein binding; hydrolase activity; ATPase activity; nucleotide binding; microtubule-severing ATPase activity; ATP binding

Biological Process: positive regulation of receptor internalization; negative regulation of synaptic transmission, glutamatergic; learning; memory; cytoplasmic microtubule organization and biogenesis
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Research Articles on Atad1

1. Yeast Msp1 and mammalian ATAD1 are conserved members of the mitochondrial protein quality control system that might promote the extraction and degradation of mislocalized TA proteins to maintain mitochondrial integrity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Atad1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with Atad1 and Brain
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