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Choline Acetyltransferase (CHAT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS807522

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple,… More details

$565 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Choline Acetyltransferase (CHAT), Polyclonal Antibody
Full Product Name
Rabbit Anti-Human Choline Acetyltransferase Polyclonal
Product Gene Name
anti-CHAT antibody
Product Synonym Names
CHAT_Human Antibody; Acetyl CoA choline O Acetyltransferase Antibody; Choline Acetylase Antibody; CLAT_Human Antibody; CMS1A Antibody; Choline Acetyltransferase Antibody: FITC; Rabbit Anti-Human Choline Acetyltransferase Polyclonal; Choline Acetyltransferase Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS807522)
FITC Conjugated Antibody: Choline Acetyltransferase (MBS807522)
Matching Pairs
Unconjugated Antibody: Choline Acetyltransferase (MBS807541)
FITC Conjugated Antibody: Choline Acetyltransferase (MBS807522)
Molecular Weight
Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
OMIM
118490
3D Structure
ModBase 3D Structure for P28329
Immunogen
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
Immunogen Species
Human
COA
A 1:1000 dilution was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Conjugate
FITC
Cellular Localization
Cytoplasm, Cytosol, Mitochondrion, Nucleus

Product Categories/Family for anti-CHAT antibody

Neuroscience; Neurotransmitter Receptors; Cell Signaling; Neurodegeneration; Alzheimer's Disease
Applications Tested/Suitable for anti-CHAT antibody
Western Blot (WB)
Application Notes for anti-CHAT antibody
Western Blot: 1:1000

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-CHAT antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Rabbit Anti-CHAT Polyclonal Antibody – Cat# MBS807522

Western Blot (WB) of anti-CHAT antibody

Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
Rabbit Anti-CHAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS807522

Product References and Citations for anti-CHAT antibody

1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.

Other Notes

Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHAT purchase

MBS807522 is an antibody targeting CHAT. Also known as Rabbit Anti-Human Choline Acetyltransferase Polyclonal. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.

NCBI GI #
218931221
NCBI GeneID
1103
NCBI Accession #
NP_001136401.1
UniProt Primary Accession #
P28329
UniProt Secondary Accession #
Q16488; Q9BQ23; Q9BQ35; Q9BQE1; A2BDF4; A2BDF5
UniProt Related Accession #
P28329
GenBank Nucleotide #
NM_001142929.1
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
UniProt Protein Name
Choline O-acetyltransferase
Protein Family
(Z)-3-hexen-1-ol acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_HUMAN
NCBI Protein Information
choline O-acetyltransferase

NCBI Summary for CHAT

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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UniProt Comments for CHAT

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: EC 2.3.1.6; Acetyltransferase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleus

Molecular Function: choline binding; choline O-acetyltransferase activity

Biological Process: acetylcholine biosynthetic process; antral ovarian follicle growth; glycerophospholipid biosynthetic process; memory; neurotransmitter secretion; phosphatidylcholine biosynthetic process; phospholipid metabolic process; response to drug; response to ethanol; response to hypoxia; response to nutrient; synaptic transmission

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
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Research Articles on CHAT

1. Data show thata the expression of choline acetyltransferase (ChAT) is reduced in the postmortem alcoholic basal forebrain in comparison to moderate drinking controls.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHAT antibody

 Reference Product  PubMed Publications
 ACHE antibody  >734 publications with CHAT and ACHE
 SLC18A3 antibody  >94 publications with CHAT and SLC18A3
 SLC5A7 antibody  >6 publications with CHAT and SLC5A7
 SLC44A1 antibody  >2 publications with CHAT and SLC44A1
 NTRK1 antibody  >2 publications with CHAT and NTRK1
 VAMP2 antibody  >1 publications with CHAT and VAMP2
 KIFAP3 antibody  >1 publications with CHAT and KIFAP3

Pathways associated with anti-CHAT antibody

 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram

Diseases associated with anti-CHAT antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2629 publications with CHAT and Nervous System Diseases
 Brain Diseases Antibodies  >1402 publications with CHAT and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1062 publications with CHAT and Neurodegenerative Diseases
 Neurobehavioral Manifestations Antibodies  >928 publications with CHAT and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >602 publications with CHAT and Disease Models, Animal
 Nerve Degeneration Antibodies  >448 publications with CHAT and Nerve Degeneration
 Cardiovascular Diseases Antibodies  >341 publications with CHAT and Cardiovascular Diseases
 Memory Disorders Antibodies  >289 publications with CHAT and Memory Disorders
 Movement Disorders Antibodies  >280 publications with CHAT and Movement Disorders
 Cognition Disorders Antibodies  >266 publications with CHAT and Cognition Disorders

Organs/Tissues associated with anti-CHAT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6073 publications with CHAT and Brain
 Eye Antibodies  >575 publications with CHAT and Eye
 Testis Antibodies  >13 publications with CHAT and Testis
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