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O-GalNAc Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808567

MBS808567 is an antibody targeting O-GalNAc. Also known as O-GalNAc Antibody, Clone 9B9: Biotin. Reactive in Species Independent. Host: Mouse. More details

$660 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
O-GalNAc, Monoclonal Antibody
Full Product Name
O-GalNAc Antibody, Clone 9B9: Biotin
Product Gene Name
Product Synonym Names
Mouse Anti-beta-O-Linked N-Acetylgalactosamine Monoclonal IgM; O-GalNAc Antibody: Biotin; GalNAC Antibody; O-GalNAC Antibody; beta-O-Linked N-Acetylgalactosamine Antibody; O-Linked N-Acetylgalactosamine Antibody; N-Acetylgalactosamine Antibody; 2-(Acetylamino)-2-deoxy-D-galactose Antibody; 2-Acetamido-2-deoxy-D-galactose Antibody; N-Acetylchondrosamine Antibody; 2-Acetamido-2-deoxy-D-galactopyranose Antibody; N-Acetyl-D-galactosamine Antibody; alpha-N-Acetylgalactosamine Antibody; N-Acetylgalactosamine-modified protein Antibody
Clonality
Monoclonal
Isotype
IgM
Clone Number
9B9
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
PEG Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for beta-O-Linked N-Acetylgalactosamine and beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: O-GalNAc Clone #9B9 (MBS808556)
Biotin Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808567)
Matching Pairs
Unconjugated Antibody: N/A (MBS808567)
Biotin Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808567)
Target
O-GalNAC
Immunogen
Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Biotin
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Applications Tested/Suitable for anti-O-GalNAc antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-O-GalNAc antibody
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-O-GalNAc antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-O-GalNAc Monoclonal Antibody – Cat# MBS808567

Western Blot (WB) of anti-O-GalNAc antibody

Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-O-GalNAc Monoclonal Antibody, Western Blot (WB) – Cat# MBS808567

Other Notes

Small volumes of anti-O-GalNAc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for O-GalNAc. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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