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Hexanoyl-Lysine Adduct (HEL) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808647

MBS808647 is an antibody targeting HEL. Also known as Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 390. Reactive in Species Independent. Host: Mouse. More details

$665 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hexanoyl-Lysine adduct (HEL), Monoclonal Antibody
Full Product Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 390
Product Gene Name
anti-HEL antibody
Product Synonym Names
Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1; Hexanoyl-Lysine adduct Antibody: ATTO 390; Hexanoyl-Lysine adduct Antibody; HEL (Hexanoyl-Lysine adduct) Antibody; HEL Antibody; HEL Adduct Antibody; Hexanoyl-Lys adduct Antibody; Hexanoyl-Lys Antibody; Hexanoyl-Lysine (HEL) adduct Antibody; Hexanoyl-Lys (HEL) Antibody; Hexanoyl Lysine adduct Antibody; Hexanoyl-Lysine adduct-modified protein Antibody; Hexanoyl-Lysine Adduct (HEL) Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D9
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: Hexanoyl-Lysine adduct Clone #5D9 (MBS808646)
ATTO 390 Conjugated Antibody: Hexanoyl-Lysine adduct Clone #5D9 (MBS808647)
Matching Pairs
Unconjugated Antibody: N/A (MBS808647)
ATTO 390 Conjugated Antibody: Hexanoyl-Lysine adduct Clone #5D9 (MBS808647)
Target
Hexanoyl-Lysine adduct
Immunogen
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
ATTO 390
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Product Categories/Family for anti-HEL antibody

Antibodies; Cancer; Oxidative Stress; Lipid peroxidation
Applications Tested/Suitable for anti-HEL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-HEL antibody
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000
FC/FACS: 1:50

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-HEL antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-HEL Monoclonal Antibody – Cat# MBS808647

Western Blot (WB) of anti-HEL antibody

Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-HEL Monoclonal Antibody, Western Blot (WB) – Cat# MBS808647

Flow Cytometry (FC/FACS) of anti-HEL antibody

Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.
Mouse Anti-HEL Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS808647

Western Blot (WB) of anti-HEL antibody

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Mouse Anti-HEL Monoclonal Antibody, Western Blot (WB) – Cat# MBS808647

Other Notes

Small volumes of anti-HEL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HEL. It may not necessarily be applicable to this product.

Protein Family
Hevein-like preproprotein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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