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Alpha Synuclein (SNCA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808916

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally,… More details

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Product Overview

Online Datasheet in PDF
Product Name
Alpha Synuclein (SNCA), Monoclonal Antibody
Full Product Name
Alpha Synuclein Antibody, Clone 3C11
Product Gene Name
anti-SNCA antibody
Product Synonym Names
Mouse Anti-Human Alpha Synuclein Monoclonal IgG1; Alpha Synuclein Antibody; Alpha Synuclein Antibody; Non-A beta component of AD amyloid Antibody; Non-A4 component of amyloid precursor Antibody; NACP Antibody; SNCA Antibody; PARK1 Antibody; PARK 1 Antibody; alphaSYN Antibody; PARK 4 antibody; PARK4 antibody; Parkinson disease familial 1 antibody; Parkinson disease (autosomal dominant; Lewy body) 4 antibody; SYN antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C11
Host
Mouse
Species Reactivity
Human; Mouse; Rat
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Binds within the 81-130 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Band at ~30 kDa is a dimer.
Preparation and Storage
Store at -20 degree C; 1 year+; Avoid freeze/thaw cycle.
Matching Pairs
Antibody: N/A (MBS808916)
Antibody: Alpha Synuclein Clone #3C11 (MBS808916)
Matching Pairs
Unconjugated Antibody: Alpha Synuclein Clone #3C11 (MBS808916)
ATTO 390 Conjugated Antibody: Alpha Synuclein Clone #3C11 (MBS808917)
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OMIM
127750
3D Structure
ModBase 3D Structure for P37840
Immunogen
Human alpha synuclein monomer
Cellular Localization
Cytoplasm; Cytosol; Membrane; Nucleus; Cell Junction; Synapse

Product Categories/Family for anti-SNCA antibody

Antibodies; Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein
Applications Tested/Suitable for anti-SNCA antibody
Western Blot (WB), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-SNCA antibody
WB: 1:1000
DB: 1:1000
ICC/IF: 1:100
ELISA: 1:1000

A 1:1000 dilution of SMC-530 was sufficient for detection of Alpha Synuclein in 15 ug of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Optimal dilutions for assays should be determined by the end user

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-SNCA antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
Mouse Anti-SNCA Monoclonal Antibody – Cat# MBS808916

Western Blot (WB) of anti-SNCA antibody

Western Blot analysis of Human Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).
Mouse Anti-SNCA Monoclonal Antibody, Western Blot (WB) – Cat# MBS808916

Western Blot (WB) of anti-SNCA antibody

Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).
Mouse Anti-SNCA Monoclonal Antibody, Western Blot (WB) – Cat# MBS808916

Product References and Citations for anti-SNCA antibody

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.

Other Notes

Small volumes of anti-SNCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SNCA purchase

MBS808916 is an antibody targeting SNCA. Also known as Alpha Synuclein Antibody, Clone 3C11. Reactive in Human; Mouse; Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SNCA. It may not necessarily be applicable to this product.

NCBI GI #
4507109
NCBI GeneID
6622
NCBI Accession #
NP_000336.1
UniProt Primary Accession #
P37840
UniProt Secondary Accession #
Q13701; Q4JHI3; Q6IAU6; A8K2A4
UniProt Related Accession #
P37840
GenBank Nucleotide #
NM_000345.3
NCBI Official Full Name
alpha-synuclein isoform NACP140
NCBI Official Synonym Full Names
synuclein alpha
NCBI Official Symbol
SNCA
NCBI Official Synonym Symbols
PD1; NACP; PARK1; PARK4
UniProt Protein Name
Alpha-synuclein
UniProt Synonym Protein Names
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Protein Family
Alpha-synuclein
UniProt Gene Name
SNCA
UniProt Synonym Gene Names
NACP; PARK1; NACP
NCBI Protein Information
alpha-synuclein

NCBI Summary for SNCA

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
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UniProt Comments for SNCA

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Research Articles on SNCA

1. This study provides the preclinical rationale for the combined blockade of PD-1/PD-L1 and C5a to restore antitumor immune responses, inhibit tumor cell growth, and improve outcomes of patients with lung cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SNCA antibody

 Reference Product  PubMed Publications
 PARK2 antibody  >1228 publications with SNCA and PARK2
 PARK7 antibody  >67 publications with SNCA and PARK7
 SNCAIP antibody  >35 publications with SNCA and SNCAIP
 UCHL1 antibody  >33 publications with SNCA and UCHL1
 APP antibody  >27 publications with SNCA and APP
 SLC6A3 antibody  >16 publications with SNCA and SLC6A3
 FYN antibody  >4 publications with SNCA and FYN
 KLK6 antibody  >1 publications with SNCA and KLK6
 SIAH2 antibody  >1 publications with SNCA and SIAH2
 UBB antibody  >1 publications with SNCA and UBB

Pathways associated with anti-SNCA antibody

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyloid Fiber Formation Pathway antibodies  Amyloid Fiber Formation Pathway Diagram
 Ectoderm Differentiation Pathway antibodies  Ectoderm Differentiation Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram

Diseases associated with anti-SNCA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1519 publications with SNCA and Nervous System Diseases
 Brain Diseases Antibodies  >1496 publications with SNCA and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1472 publications with SNCA and Neurodegenerative Diseases
 Movement Disorders Antibodies  >1321 publications with SNCA and Movement Disorders
 Parkinsonian Disorders Antibodies  >1215 publications with SNCA and Parkinsonian Disorders
 Parkinson Disease Antibodies  >1176 publications with SNCA and Parkinson Disease
 Lewy Body Disease Antibodies  >336 publications with SNCA and Lewy Body Disease
 Disease Models, Animal Antibodies  >219 publications with SNCA and Disease Models, Animal
 Nerve Degeneration Antibodies  >182 publications with SNCA and Nerve Degeneration
 PARKINSON DISEASE 1, AUTOSOMAL DOMINANT Antibodies  >70 publications with SNCA and PARKINSON DISEASE 1, AUTOSOMAL DOMINANT

Organs/Tissues associated with anti-SNCA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1083 publications with SNCA and Brain
 Nerve Antibodies  >1054 publications with SNCA and Nerve
 Blood Antibodies  >138 publications with SNCA and Blood
 Embryonic Tissue Antibodies  >29 publications with SNCA and Embryonic Tissue
 Kidney Antibodies  >21 publications with SNCA and Kidney
 Eye Antibodies  >19 publications with SNCA and Eye
 Skin Antibodies  >15 publications with SNCA and Skin
 Heart Antibodies  >15 publications with SNCA and Heart
 Vascular Antibodies  >15 publications with SNCA and Vascular
 Bone Antibodies  >11 publications with SNCA and Bone
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