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Alpha Synuclein (SNCA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808974

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally,… More details

$575 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Alpha Synuclein (Snca), Monoclonal Antibody
Full Product Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 594
Product Gene Name
anti-Snca antibody
Product Synonym Names
Mouse Anti-Mouse Alpha Synuclein Monoclonal IgG1; Alpha Synuclein Antibody: ATTO 594; Alpha Synuclein Antibody; Non-A beta component of AD amyloid Antibody; Non-A4 component of amyloid precursor Antibody; NACP Antibody; SNCA Antibody; PARK1 Antibody; PARK 1 Antibody; alphaSYN Antibody; PARK 4 antibody; PARK4 antibody; Parkinson disease familial 1 antibody; Parkinson disease (autosomal dominant; Lewy body) 4 antibody; SYN antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4F1
Host
Mouse
Species Reactivity
Human; Mouse; Rat
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Binds multiple linear epitopes within human alpha synuclein: 21-70 aa, 61-90 aa, and 101-140 aa, determined using Dot blot and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Band at ~40 kDa is a trimer.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: Alpha Synuclein Clone #4F1 (MBS808970)
ATTO 594 Conjugated Antibody: Alpha Synuclein Clone #4F1 (MBS808974)
Matching Pairs
Unconjugated Antibody: N/A (MBS808974)
ATTO 594 Conjugated Antibody: Alpha Synuclein Clone #4F1 (MBS808974)
Molecular Weight
12,345 Da
3D Structure
ModBase 3D Structure for O55042
Target
Alpha Synuclein
Immunogen
Mouse alpha synuclein aggregate
Immunogen Species
Mouse
Cellular Localization
Cytoplasm; Cytosol; Membrane; Nucleus; Cell Junction; Synapse
Conjugate
ATTO 594
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

Product Categories/Family for anti-Snca antibody

Antibodies; Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein
Applications Tested/Suitable for anti-Snca antibody
Western Blot (WB), Dot Blot (DB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-Snca antibody
WB: 1:1000
DB: 1:1000
IHC: 1:200
ICC/IF: 1:100
ELISA: 1:1000

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Snca antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
Mouse Anti-SNCA Monoclonal Antibody – Cat# MBS808974

Western Blot (WB) of anti-Snca antibody

Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).
Mouse Anti-SNCA Monoclonal Antibody, Western Blot (WB) – Cat# MBS808974

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Snca antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.
Mouse Anti-SNCA Monoclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS808974

Product References and Citations for anti-Snca antibody

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.

Other Notes

Small volumes of anti-Snca antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Snca purchase

MBS808974 is an antibody targeting Snca. Also known as Alpha Synuclein Antibody, Clone 4F1: ATTO 594. Reactive in Human; Mouse; Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Snca. It may not necessarily be applicable to this product.

NCBI GI #
109638747
NCBI GeneID
20617
NCBI Accession #
NP_001035916.1
UniProt Primary Accession #
O55042
UniProt Secondary Accession #
Q3U130; Q9CXF8; Q9EQC3; Q9QUR3
UniProt Related Accession #
O55042
GenBank Nucleotide #
NM_001042451.2
NCBI Official Full Name
alpha-synuclein
NCBI Official Synonym Full Names
synuclein, alpha
NCBI Official Symbol
Snca
NCBI Official Synonym Symbols
NACP; alphaSYN; alpha-Syn
UniProt Protein Name
Alpha-synuclein
UniProt Synonym Protein Names
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Protein Family
Alpha-synuclein
UniProt Gene Name
Snca
UniProt Synonym Gene Names
Syn; NACP
NCBI Protein Information
alpha-synuclein

UniProt Comments for Snca

May be involved in the regulation of dopamine release and transport.

Research Articles on Snca

1. alpha-synuclein levels can be reduced in neurons without impairing (or improving) mitochondrial bioenergetics or distribution

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Snca antibody

 Reference Product  PubMed Publications
 Park2 antibody  >1228 publications with Snca and Park2
 Lrrk2 antibody  >197 publications with Snca and Lrrk2
 Park7 antibody  >67 publications with Snca and Park7
 Sncaip antibody  >35 publications with Snca and Sncaip
 App antibody  >27 publications with Snca and App
 Slc6a3 antibody  >16 publications with Snca and Slc6a3
 Fyn antibody  >4 publications with Snca and Fyn
 Klk6 antibody  >1 publications with Snca and Klk6

Pathways associated with anti-Snca antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram

Diseases associated with anti-Snca antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1519 publications with Snca and Nervous System Diseases
 Brain Diseases Antibodies  >1496 publications with Snca and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1472 publications with Snca and Neurodegenerative Diseases
 Movement Disorders Antibodies  >1321 publications with Snca and Movement Disorders
 Parkinsonian Disorders Antibodies  >1215 publications with Snca and Parkinsonian Disorders
 Parkinson Disease Antibodies  >1176 publications with Snca and Parkinson Disease
 Lewy Body Disease Antibodies  >336 publications with Snca and Lewy Body Disease
 Disease Models, Animal Antibodies  >219 publications with Snca and Disease Models, Animal
 Nerve Degeneration Antibodies  >182 publications with Snca and Nerve Degeneration
 PARKINSON DISEASE 1, AUTOSOMAL DOMINANT Antibodies  >70 publications with Snca and PARKINSON DISEASE 1, AUTOSOMAL DOMINANT

Organs/Tissues associated with anti-Snca antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1083 publications with Snca and Brain
 Embryonic Tissue Antibodies  >29 publications with Snca and Embryonic Tissue
 Eye Antibodies  >19 publications with Snca and Eye
 Skin Antibodies  >15 publications with Snca and Skin
 Liver Antibodies  >11 publications with Snca and Liver
 Bone Antibodies  >11 publications with Snca and Bone
 Lung Antibodies  >10 publications with Snca and Lung
 Sympathetic Ganglion Antibodies  >6 publications with Snca and Sympathetic Ganglion
 Olfactory Mucosa Antibodies  >3 publications with Snca and Olfactory Mucosa
 Prostate Antibodies  >3 publications with Snca and Prostate
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